标题：宫颈癌组织中Twist与E-cadherin蛋白的表达及其意义

      作者：张荣荣，王 红，张 萍
    (上海交通大学医学院附属新华医院妇科，上海 200092)

      卷次： 2013年24卷8期

      【摘要】 目的 研究Twist与E-cadherin蛋白在宫颈炎、宫颈上皮内瘤变及宫颈癌组织中的表达水平，分析
其在宫颈癌中的作用。方法 选取宫颈炎（20例）、宫颈上皮内瘤变（40例）和宫颈鳞状细胞癌（40例）的石蜡标
本为研究对象，采用免疫组织化学方法检测Twist与E-cadherin蛋白在上述宫颈组织中的表达情况，分析其临床
意义。结果 ①宫颈细胞胞质、胞核均可见Twist蛋白表达，以胞质表达更明显，其阳性率随恶性程度增加而逐
渐增多；E-cadherin蛋白主要在细胞膜表达，其阳性率随恶性程度增加而降低。Twist和E-cadherin蛋白在宫颈
炎、CIN及宫颈鳞癌组织中表达差异有统计学意义(P<0.05)。②在宫颈鳞癌组织中，Twist蛋白在有淋巴结转移
的病例中高表达，E-cadherin蛋白仅在部分无淋巴结转移的病例中少量表达。Twist和E-cadherin蛋白阳性率在
不同淋巴结转移差异有统计学意义（P<0.05），而在不同FIGO分期、不同肿瘤分化程度以及不同淋巴血管间隙浸
润的组织中差异无统计学意义（P>0.05）。经Spearman相关性分析，Twist蛋白与E-cadherin蛋白存在负相关性
(r=-0.561，P<0.05)。结论 在宫颈癌变过程中Twist蛋白表达逐渐增加，E-cadherin蛋白表达逐渐降低，其介导的
上皮间质化及两种蛋白的表达水平有助于判断肿瘤分化程度及转移潜能。

      【关键词】 Twist；E-cadherin蛋白；宫颈癌

      【中图分类号】 R737.33 【文献标识码】 A 【文章编号】 1003—6350（2013）08—1105—03


Expression of Twist and E-cadherin protein in cervical cancer tissue and their significance.
ZHANG Rong-rong,
WANG Hong, ZHANG Ping. Department of Gynecology, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University
School of Medcine, Shanghai 200092, CHINA

【Abstract】 Objective To investigate the expression levels of Twist and E-cadherin protein in cervicitis, cer-
vical intraepithelial neoplasia and cervical cancer, and to analyze their roles in cervical cancer. Methods Paraffin-em-
bedded samples of cervicitis (20 cases), cervical intraepithelial neoplasia (40 cases) and cervical squamous cell carci-
noma (40 cases) were the research objects. The expression of Twist and E-cadherin protein was detected with immuno-
histochemical method in the cervical tissues, and the clinical significance was analyzed. Results ① Cervical cell cy-
toplasm, nuclei were detected with the expression of Twist protein, especially in cytoplasm, and the positive rate in-
creased with the aggravation of malignancy. E-cadherin protein was mainly expressed in the cell membrane, and the
positive rate decreased with increasing malignancy. Twist and E-cadherin protein expression in cervicitis, CIN and cer-
vical squamous cell carcinoma has significant difference (P<0.05) in cervical squamous cell carcinoma, Twist protein
in the high expression of lymph nodes metastasis, E-cadherin protein only in part without lymph node metastasis cases
less expression. The positive rates of Twist and E-cadherin protein in different lymph node metastasis had significant
differences (P<0.05), while no statistically significant difference was found in different FIGO stage, tumor differentia-
tion degree and different organizations of different lymph vascular space invasion (P>0.05). Spearman correlation analysis
showed a negative correlation between Twist protein and E-cadherin protein (r=-0.561, P<0.05). Conclusion In the develop-
ment of cervical carcinoma, Twist protein expression increased gradually, and the expression of E-cadherin protein de-
creased gradually. Epithelial mesenchymal mediated by the two proteins and their expression levels help determine the
degree of tumor differentiation and metastasis.

      【Key words】 Twist; E-cadherin protein; Cervical cancer