标题：血红素加氧酶-1在白藜芦醇拮抗阿霉素致淋巴瘤裸鼠动脉内皮凋亡中的作用

      作者：顾 俊 1，胡 伟 1，宋芝萍 1，陈跃光 1，刘 旭 2，张大东 1*
    (1.上海瑞金医院集团闵行区中心医院心内科，上海 201100；
2.上海交通大学附属胸科医院心内科，上海 200030)

      卷次： 2012年23卷23期

      【摘要】 目的 评价血红素加氧酶-1 (HO-1)在白藜芦醇(RES)拮抗阿霉素(DOX)导致淋巴瘤裸鼠动脉内皮
细胞凋亡中的作用。方法 40只雄性Balb/c裸鼠经皮下接种Raji细胞建立淋巴瘤模型后随机分成四组：对照组
(n=10)，DOX组(n=10)，DOX+RES组(n=10)和DOX+RES+HO-1抑制剂锡原卟啉(ZnPP)组(n=10)。测定颈动脉组
织HO-1、Caspase 3活性。免疫组化方法检测颈动脉HO-1，Bcl-2和Bax表达。应用TUNEL方法检测颈动脉组
织细胞凋亡。结果 DOX+RES组较DOX组颈动脉组织HO-1表达和酶活性明显升高(P<0.01)，Bcl-2表达升高
(P<0.01)，Bax表达降低(P<0.01)，Caspase-3活性降低(P<0.01)。TUNEL结果提示DOX+RES组较DOX组的动脉
内皮凋亡细胞数量明显减少(P<0.01)。RES的上述动脉内皮保护作用被ZnPP明显抑制(P<0.05)。结论 HO-1
参与RES对于DOX致淋巴瘤裸鼠动脉内皮细胞凋亡的拮抗作用。

      【关键词】 白藜芦醇；阿霉素；内皮细胞；凋亡；血红素氧合酶；淋巴细胞瘤裸鼠

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2012）23—018—04<

br>Role of HO-1 in the protective effect of resveratrol on doxorubicin-induced artery endothelial apoptosis in lym-
phoma nude mice.
GU Jun 1, HU Wei 1, SONG Zhi-ping 1, CHEN Yue-guang 1, LIU Xu 2, ZHANG Da-dong 1*. 1. Depart-
ment of Cardiology, Shanghai Minhang District Central Hospital, Ruijin Hospital Group, Shanghai 201100, CHINA; 2. De-
partment of Cardiology, Shanghai Chest Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200030, CHINA

【Abstract】 Objective To study the role of HO-1 in the protective effect of resveratrol (RES) against artery
endothelial apoptosis induced by doxorubicin (DOX) in lymphoma nude mice. Methods Forty male Balb/C nude
mice after Raji cells injection were randomly divided into four groups (n=10 for each group): the control group, the
DOX group, the DOX+RES gorup; DOX+RES+ZnPP group. The activity of HO-1 and Caspase 3 were determined,
and the expression of HO-1, Bcl-2 and Bax protein were observed by immunohistochemisty. Furthermore, endothelial
apoptosis was evaluated by TUNEL. Results In the DOX+RES group, the enzymatic activity and HO-1 expression
were enhanced compared with those in the DOX group (P<0.01), with significantly increased expression of Bcl-2 (P<
0.01), decreased expression of Bax (P<0.01), and decreased activity of Caspase-3 (P<0.01). TUNEL results revealed
that the number of artery endothelial cells with apoptosis was significantly less in the DOX+RES group than the DOX
·实验研究·doi:10.3969/j.issn.1003-6350.2012.23.007 group (P<0.01). The protective effects of RES were significantly inhibited by ZnPP in the DOX+RES+ZnPP group
(P<0.05). Conclusion HO-1 plays a role in the protective effect of RES on DOX-induced artery endothelial apopto-
sis in lymphoma nude mice.

      【Key words】 Resveratrol; Doxorubixin; Endothelial cell; Apoptosis; Heme oxygenase-1; Lymphoma nude mice