标题：siRNA沉默膀胱癌T24细胞DNA甲基转移酶1(DNMT1)表达的研究

      作者：罗 欣 1，李杰贤 1，林宇峰 1，邹 戈 1，姚伟祥 1，谢文练 2
    （1.番禺中心医院泌尿外科，广东 广州 511400；
2.中山大学附属孙逸仙医院泌尿外科，广东 广州 510120)

      卷次： 2012年23卷20期

      【摘要】 目的 研究 siRNA对膀胱癌细胞T24 DNA甲基转移酶1 (DNMT1)表达的沉默作用。方法 将筛
选出的 siRNA转染入膀胱癌 T24细胞分为空白组、脂质体组、阴性对照组和 siRNA组，分别运用 RT-PCR、
Western blot检测转染后不同时段DNMT1 mRNA水平、蛋白水平变化。结果 筛选出的 siRNA转染膀胱癌T24
细胞DNMT1 24 h开始出现mRNA减少，24 h、48 h、72 h分别降至（66.05±4.87）%、（39.36±4.53）%和（40.86±
4.81）%（P<0.05）。转染后 24 h、48 h、72 h蛋白水平分别降至（90.21±3.68）%、（71.45±3.44）%和（67.74±3.38）%
（P<0.05）。结论 siRNA能有效地沉默膀胱癌细胞T24 DNMT1的表达。

      【关键词】 DNA甲基转移酶1(DNMT1)；小分子RNA (siRNA)；膀胱癌细胞T24；逆转录聚合酶链反应

      【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350（2012）20—001—02<

br>Effect of siRNA on the expression of DNMT1 in bladder cancer cell line T24.
LUO Xin 1，LI Jie-xian 1, LIN
Yu-feng 1, ZOU ge 1, YAO Wei-xiang 1, XIE Wen-lian 2. 1. Department of Urology, the People's Hospital of Panyu,
Guangzhou 511400, Guangdong, CHINA; 2. Department of Urology, the Second Affiliated Hospital of Sun Yat-sen
University, Guangzhou 510120, Guangdong, CHINA

【Abstract】 Objective To study the effect of siRNA on the expression of DNA Methyltransferase-1 (DN-
MT1) in human bladder cancer cell line T24. Methods The siRNA screened was transfected into bladder carcinoma
cells line T24. Four groups were enrolled in this study: blank group, liposomes group, negative control group, siRNA
group. The expression levels of DNMT1 mRNA were detected by RT-PCR and Western blot at different times after
transfection. Results The expression level of DNMT1 was found to start reducing 24 h after transfection. 24 h, 48 h,
and 72 h after transfection, the levels of DNMT1 were reduced to (66.05±4.87)%, (39.36±4.53)% and (40.86±4.81)%,
and those of protein were reduced to (90.21 ± 3.68)% , (71.45 ± 3.44)% and (67.74 ± 3.38)% , P<0.05, respectively.
Conclusion The siRNA can efficiently silencing the expression of DNMT1 gene in human bladder cancer cell line T24.

      【Key words】 DNMT1; siRNA; Bladder cancer cells line T24; RT-PCR
·论 著·