标题:西安市2 841例女性人乳头瘤病毒感染状况及基因型分析
作者:杨瑞利,翟华丽,沈建军王飞霞
(长安医院检验科,陕西 西安 710016)
卷次:
2016年27卷6期
【摘要】 目的 分析西安市女性宫颈上皮细胞人乳头瘤病毒(HPV)感染状况和基因亚型分布特点,以了解本
地区HPV感染现状,为宫颈癌的防治提供参考依据。方法 采用聚合酶链反应(PCR)-反向点杂交技术,对2013年
1月至2014年12月来我院妇科就诊的2 841例女性患者的宫颈黏液进行HPV分型检测,分析HPV在女性人群中的
感染率、基因型分布和年龄分布特点。结果 2 841例样本中HPV阳性样本639例,总感染率为22.49%。检出的高危
亚型中前3种型别依次为HPV52型、58型、16型;低危型感染以HPV6型最高,其次是HPV11型。感染者以≤20岁年
龄组最高,不同年龄段的HPV感染率差异有统计学意义(χ2=61.87,P<0.01)。结论 该地区女性HPV感染常见亚型为
HPV52型、58型、16型,年轻女性感染率最高,HPV基因的准确分型对宫颈癌的早期预防和诊断有重要意义。
【关键词】 西安;人乳头瘤病毒;基因型;分布
【中图分类号】 R711.7 【文献标识码】 A 【文章编号】 1003—6350(2016)06—0885—03
Infection status and genotype distributions of human papillomavirus infection in 2 841 women of Xi'an, Shaanxi,
CHINA.
YANG Rui-li, ZHAI Hua-li, SHEN Jian-jun, WANG Fei-xia. Department of Clinical Laboratory, the Hospital of
Chang'an, Xi'an 710016, Shaanxi, CHINA
【Abstract】 Objective To analyze the infection status and gene distribution of human papillomavirus (HPV) in-
fection in Xi’an, so as to provide data support for the prevention and control of cervical cancer and the clinical applica-
tion of cervical cancer vaccine. Methods HPV genotype of cervical mucus of 2 841 screening women in our hospital
from January 2013 to November 2014 was detected by polymerase chain reaction (PCR) and reverse dot blot hybridiza-
tion. The infection rate, genotype and age distribution in the females were analyzed. Results Among the 2 841 women,
639 women were infected by HPV, and the total infection rate of HPV was 22.49%. The first three high-risk HPV sub-
types were HPV52, 58, 16. The infection rate of HPV6 was highest in the low-risk genotypes, followed by HPV11. The
infection rate of≤20-year-old group was significantly higher than that of the other groups. There was a significant dif-
ference in the infection rate among the various age groups (χ2=61.87, P<0.01). Conclusion HPV52, HPV58 and HPV16
were the major subtypes in the women of Xi'an. The infection rate of young women was higher than that of the other age
groups. The detection of HPV genotypes is very meaningful in early prevention and diagnosis of cervical cancer.
【Key words】 Xi'an; Human papillomavim; Genotype; Distribution
·论 著·
6350.2016.06.009
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