标题：MMP-2、MMP-9在膀胱移行细胞癌组织中的表达及其临床意义

      作者：王碧玉 1，黄燕妮 2，羊文芳 3
    (1.海南省农垦三亚医院检验科，海南 三亚 572000；
2.海南医学院热带医学与检验医学院，海南 海口 570102；
3.儋州市第一人民医院检验科，海南 儋州 571700)

      卷次： 2016年27卷6期

      【摘要】 目的 观察膀胱移行细胞癌组织中基质金属蛋白酶2 (MMP-2)和基质金属蛋白酶9 (MMP-9)的表达
情况，并探讨其表达的临床意义。方法 选取海南省农垦三亚医院2012年1月至2015年1月收治的30例膀胱移行
细胞癌组织标本作为观察组，另选取非膀胱肿瘤手术中切术的正常膀胱组织标本30例作为对照组，对所有标本黏
膜中MMP-2、MMP-9的表达情况与表达强度通过免疫组化SP法联合高清晰度彩色医学图文分析系统进行检测，
分析其表达的临床意义。结果 观察组标本中的MMP-2、MMP-9的表达比对照组标本的表达明显增高，差异均
有统计学意义(P<0.05)，且MMP-2、MMP-9在正常膀胱上皮呈阴性表达；观察组标本中的MMP-2、MMP-9呈现高
表达状态，且表达强度随着疾病分期、分级的增高而逐渐增高，差异均有统计学意义(P<0.05)。结论 在膀胱癌侵
袭转移过程中，存在MMP-2、MMP-9的参与，在膀胱移行细胞癌中MMP-2、MMP-9的表达对其预后判断具有非
常重要的参考价值。

      【关键词】 膀胱移行细胞癌；基质金属蛋白酶2；基质金属蛋白酶9；表达

      【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350（2016）06—0877—04


Expression of MMP-2, MMP-9 in human transitional cell carcinoma of bladder and their clinical significance.
WANG Bi-yu 1, HUANG Yan-ni 2, YANG Wen-fang 2 .
1. Department of Clinical Laboratory, Hainan Provincial Nongken
Sanya Hospital, Sanya 572000, Hainan, CHINA; 2. College of Tropical Medicine and Laboratory Medicine, Hainan Medical
University, Haikou 570102, Hainan, CHINA; 3. Department of Clinical Laboratory, the First People's Hospital of Danzhou,
Danzhou 571700, Hainan, CHINA

【Abstract】 Objective To observe the expression of matrix metalloproteinases 2 (MMP-2) and matrix metallopro-
teinase 9 (MMP-9) in human transitional cell carcinoma of bladder and discuss the clinical significance. Methods Thirty
tissue samples of transitional cell carcinoma of bladder were collected in Hainan Provincial Nongken Sanya Hospital
from Jan. 2012 to Jan. 2015 (the observation group). Another 30 cases of normal bladder tissues were selected as the con-
trol group. Immunohistochemical SP method and high-definition color medical graphic analysis system were used to de-
tect the expression of MMP-2 and MMP-9. The clinical significance of the expression was analyzed. Results The ex-
pression of MMP-2 and MMP-9 in the observation group was significantly higher than that in the control group (P<
0.05), and the expression of MMP-2 and MMP-9 in normal bladder epithelium were negative. In the observation group,
MMP-2 and MMP-9 presents a high expression, and the expression strength increased gradually with the increase of the
grade and stage of disease (P<0.05). Conclusion MMP-2 and MMP-9 participate the process of invasion and metasta-
sis of bladder cancer, and the expression of MMP-2 and MMP-9 in transitional cell carcinoma of bladder of provides im-
portant reference for the evaluation of the prognosis.

      【Key words】 Transitional cell carcinoma of bladder; Matrix metalloproteinases 2 (MMP-2); Matrix metallopro-
teinases 9 (MMP-9); Expression
·论 著·
6350.2016.06.006