标题：监测血浆EBV-DNA含量在鼻咽癌诊疗中的意义

      作者：柳文菊，杜 昆
    （长江大学附属第一人民医院检验科，湖北 荆州 434000）

      卷次： 2012年23卷18期

      【摘要】 目的 探讨鼻咽癌患者放化疗过程中血浆EBV-DNA含量在临床诊疗中的意义。方法 采用荧光
定量PCR法检测50例NPC患者在不同的诊疗阶段血浆EBV-DNA的含量，同时设50例健康体检者为阴性对照
组。结果 50例NPC组中 EBV-DNA检测阳性率为 92.0%（46/50），健康对照组阳性率为 8.0%（4/50）；46例
EBV-DNA阳性的患者中，26例患者从第一周治疗开始，DNA水平就开始明显下降，至治疗结束前已检测不到
DNA的复制；14例患者开始几周DNA水平变化并不明显，至治疗结束前，基本检测不到DNA的复制；6例患者
在治疗过程中均可检测到较高水平的EBV-DNA。结论 采用荧光定量PCR方法检测NPC患者血浆中游离的
EBV-DNA是一种敏感可靠的方法，对于NPC的早期诊断及预后具有非常重要的临床意义。

      【关键词】 荧光定量PCR；鼻咽癌；EBV-DNA；血浆

      【中图分类号】 R739.63 【文献标识码】 A 【文章编号】 1003—6350（2012）18—007—03<

br>Clinical significance of plasma Epstein-Bar virus DNA concentration in the diagnosis and treatment of
nasopharyngeal carcinoma.
LIU Wen-ju, DU Kun. Department of Clinical Laboratory, the First Affiliated Hospital of
Yangtze University, Jingzhou 434000, HuBei, CHINA

【Abstract】 Objective To investigate the clinical significance of plasma Epstein-Bar virus DNA
(EBV-DNA) concentration in the diagnosis and treatment of nasopharyngeal carcinoma (NPC). Methods Fluores-
cence quantitative PCR was used to determine the content of EBV-DNA in blood serum in 56 patients with NPC (the
study group) and 50 healthy individuals (the control group). Results The positive rate of EBV-DNA was 92% (46/
50) in the study group and 8% (4/50) in the control group. Among the 46 patients detected positive in the study group,
26 patients were detected with significantly decreased EBV-DNA at the first week of treatment, and with no
EBV-DNA at the end of the treatment. Fourteen patients were detected with high levels of EBV-DNA at the initial
weeks of treatment, but with no EBV-DNA at the end of the treatment. Six patients were found to maintain a high lev-
els of EBV-DNA throughout the treatment. Conclusion Determination of EBV-DNA by fluorescence qualitative
PCR in blood serum is reliable and sensitive, which has high clinical value in the diagnosis and treatment of NPC.

      【Key words】 Fluorescence qualitative PCR; Nasopharyngeal carcinoma; EBV-DNA; Plasma