标题:牛耳枫药材中总黄酮含量测定
作者:李海龙,刘明生,张俊清,符乃光,陈 峰*
(海南医学院药学院 海南省热带药用植物研究开发重点实验室,海南 海口 571101)
卷次:
2012年23卷15期
【摘要】 目的 建立测定牛耳枫药材中总黄酮的含量方法。方法 以芦丁为对照,显色后采用分光光度法
于510 nm处测定总黄酮含量。结果 总黄酮在7.84~47.04 ?g/ml (r=0.999 8)范围内线性关系良好;总黄酮的平
均回收率为98.51%,RSD=2.48%。结论 建立的方法可用于牛耳枫总黄酮的测定。
【关键词】 牛耳枫;分光光度法;总黄酮;含量;测定
【中图分类号】 R28 【文献标识码】 B 【文章编号】 1003—6350(2012)15—106—03<
br>Determination of total flavonoids in Daphniphylium calycinum.
LI Hai-long, LIU Ming-sheng, ZHANG Jun-qing,
FU Nai-guang, CHEN Feng. Key Laboratory of R&D on Tropical Medicinal Plants, College of Pharmacy, Hainan
Medical University, Haikou 571101, Hainan, CHINA
【Abstract】 Objective To establish the methods for the determination of total flavonoids in Daphniphylium
calycinum. Methods The total flavonoids was determined by visable spectrophotometry with the detection wave-
length of 510 nm after color development reaction, using rutin as the control. Results The standard curve was linear
in the range of 7.84~47.04 ?g/mL for total flavonoids (r=0.999 8), and the average recovery of total flavonoids was
98.51% with RSD of 2.48%. Conclusion The method established can be used to determine the content of total flavo-
noids in Daphniphylium calycinum and to control the quality of Daphniphylium calycinum.
【Key words】 Daphniphylium calycinum; Spectrophotometry; Total flavonoids; Content; Determination
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