标题：高压氧对脑出血大鼠脑内HIF-1α表达和脑水肿的影响

      作者：王爱岳 1，李 强 2，周治平 1，许琼冠 2
    (1.中南大学湘雅医学院附属海口医院神经内科，海南 海口 570208；
2.海南省农垦总医院神经外科，海南 海口 570311)

      卷次： 2012年23卷12期

      【摘要】 目的 探讨高压氧对血肿周边脑组织中HIF-1α蛋白表达和脑水肿程度的影响。方法 SD大鼠88
只随机分为假手术对照组(sham组，8只)、脑出血组(ICH组)和脑出血+高压氧治疗组(HBO治疗组)。ICH组和
HBO治疗组又分为6 h组、24 h组、3 d组、7 d组和14 d组，每组8只。采用自体血脑内注射法建立脑出血动物模
型，干湿比重法测定脑组织含水量，以免疫组化的方法检测血肿周边脑组织中HIF-1α蛋白表达情况，同时观察高
压氧对脑水肿和HIF-1α表达的影响。结果 ICH组和HBO治疗组与 sham组比较，脑组织含水量明显增加，差异
有统计学意义(P<0.05)。HBO治疗组与 ICH组大鼠脑组织含水量第14天时比较差异有统计学意义(P<0.05)。与
sham组比较，ICH组和HBO治疗组HIF-1α表达明显增加，差异有统计学意义(P<0.05)。HBO治疗组与 ICH组大
鼠脑组织中HIF-1α表达第14天时比较，差异有统计学意义(P<0.05)。ICH组HIF-1α蛋白表达与脑水肿的程度成
正相关(P<0.01)；HBO治疗组HIF-1α蛋白表达与脑水肿的程度无相关性(P>0.05)。结论 HIF-1α在血肿周边脑
组织中的表达与脑水肿的形成和发展有关，高压氧可抑制HIF-1α蛋白的表达并减轻脑水肿。

      【关键词】 缺氧诱导因子-1α；高压氧；脑出血；脑水肿

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350(2012)12—015—03<

br>Effect of hyperbaric oxygen on HIF-1α expression and brain edema in rat with intracerebral hemorrhage.
WANG Ai-yue 1, LI Qiang 2, ZHOU Zhi-ping 1, XU Qiong-guan 2.
1. Department of Neurology, Haikou Hospital Affiliated
to Xiangya School of Medicine, Central South University, Haikou 570208, Hainan, CHINA; 2. Department of
Neurosurgery, Hainan Provincial Hospital of General Bureau of Agriculture, Haikou 570311, Hainan, CHINA

【Abstract】 Objective To investigate the effect of hyperbaric oxygen on HIF-1α expression and brain edema
in rats with intracerebral hemorrhage. Methods Eighty-eight SD rats were randomly divided into sham group, ICH
group and HBO group. Then ICH group and HBO group were divided into 6 h, 24 h, 3 d, 7 d and 14 d subgroups. Im-
munohistochemistry was applied to detect the levels of HIF-1α. The water content in rat brains was detected to deter-
mine the effects of HBO on the expression of HIF-1α in brain after intracerebral hemorrhage. Results Compared
with sham group, the water content in ICH group and HBO group were significantly increased (P<0.05), and the lev-
els of HIF-1α were also significantly increased (P<0.05). There were statistically significant difference in the levels of
HIF-1α between ICH group and HBO group on the 14th day after intracerebral hemorrhage (P<0.05). There were a
positive correlation between the levels of water content and HIF-1α labeling index in ICH group (P<0.05), while a
negative correlation in HBO group (P>0.05). Conclusion The expression of HIF-1α may be involved in the forma-
tion of brain edema after ICH. HBO retreatment may inhibit the expression of HIF-1α and reduce the formation of
brain edema.

      【Key words】 Hypoxia inducible factor-1α; Hyperbaric oxygen; Intracerebral hemorrhage; Brain edema
·实验研究·