标题：阿魏酸钠对神经病理性疼痛大鼠脊髓Bcl-2和Bax的影响

      作者：解小丽 1，周青山 2，程 周 1
    (1.中山市人民医院麻醉科，广东 中山 528403；
2.武汉大学人民医院麻醉科，湖北 武汉 430060)

      卷次： 2012年23卷8期

      【摘要】 目的 观察阿魏酸钠对神经病理性疼痛大鼠脊髓中Bcl-2和Bax表达的影响，探讨阿魏酸钠对脊
髓的保护作用。方法 成年雄性Wistar大鼠随机分为两组：A组(n=30)为生理盐水组，结扎左侧坐骨神经后每
天腹腔注入 2 ml生理盐水；B组(n=30)为阿魏酸钠组，结扎坐骨神经后向腹腔注入 2 ml阿魏酸钠，100 mg· kg-1·
d-1，连续 7 d。分别在术前及术后 6 h、1 d、3 d、7 d进行行为学测定，记录缩足阈值，检测脊髓中Bcl-2和Bax的
表达，采用TUNEL法观察脊髓神经元凋亡。结果 两组大鼠坐骨神经结扎后均可诱发出神经病理性疼痛；凋
亡神经元和Bcl-2和Bax阳性细胞均于术后 6 h开始出现，并于术后 1 d开始迅速增加，术后 3 d达峰值。与生
理盐水组相比较，阿魏酸钠组大鼠脊髓组织中神经细胞凋亡指数和Bax表达水平显著降低，而Bcl-2的表达
水平却显著增加(P<0.05)。结论 阿魏酸钠可能通过促进Bcl-2表达，抑制Bax表达，从而抑制了大鼠脊髓神经
元凋亡，对脊髓有保护作用。

      【关键词】 阿魏酸钠；脊髓保护；Bcl-2；Bax

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350(2012)08—016—03<

br>Influence of sodium ferulate on the expression of Bcl-2 and Bax in the spinal cord neurons in rats with
neuropathic pain.
XIE Xiao-li 1, ZHOU Qing-shan 2, CHEN Zhou 1. 1. Department of Anesthesiology, People's Hospital
of Zhongshan City, Zhongshan 528403, Guangdong, CHINA; 2. Department of Anesthesiology, Wuhan University Renmin
Hospital, Wuhan 430060, Hubei, CHINA

【Abstract】 Objective To observe the influence of sodium ferulate (SF) on the expression of Bcl-2 and Bax
in spinal cord neurons in rats with neuropathic pain, and to study the protective effects of SF on the spinal cord neu-
rons in rats. Methods Sixty healthy adult male wistar rats were simply randomized into two groups: group A (n=30)
and group B (n=30). The left sciatic nerves of the rats were ligated under chloral hydrate anesthesia, then the rats were
given 2 ml normal saline (group A) and 100 mg· kg-1· d-1 SF (group B), which was dissolved in 2 ml normal saline re-
spectively after operation. All the patients were tested for the time of paw withdraw by using the von Frey test before
surgery, 6 hours, 1 day , 3 days, and 7 days after surgery. Apoptosis of spinal cord neurons was detected with the TU-
NEL method. Changes of Bcl-2 and Bax expression in the spinal cord were detected through the immunohistochemi-
cal methods and image analysis. Results Mechanical allodynia developed at 6 hours after sciatic nerve ligation. The
expression of neuronal apoptosis, Bcl-2 and Bax were first detected in spinal cord at 6 hours after injury and thereaf-
ter, reaching the peak at 3 days. The spinal neuronal apoptotic index and the expression of Bax in group B was signifi-
cantly lower than that in group A. However, the expression of Bcl-2 in group B was significantly higher than that in
group A (P<0.05). Conclusion Through promoting the expression of Bcl-2 and inhibiting the expression of Bax, so-
dium ferulate can effectively inhibit spinal neuronal apoptosis and protect spinal cord neurons in rats,

      【Key words】 Sodium ferulate; Spinal cord protection; Bcl-2; Bax