标题：人和大鼠胸主动脉成纤维细胞的原代培养

      作者：鲍荣辉，周 军*，周 畅，姚 志，廖 磊
    （三峡大学第一临床医学院宜昌市中心人民医院超声科，湖北 宜昌 443000）

      卷次： 2012年23卷6期

      【摘要】 目的 培养成纤维细胞，建立不同种属来源的胸主动脉成纤维细胞的培养方法并比较其形态差
异。方法 用消化法和贴壁法分离不同来源的成纤维细胞，利用差速培养法纯化成纤维细胞，并对所培养的细
胞进行倒置显微镜观察和苏木素-伊红染色，观察成纤维细胞形态，并对培养细胞行波形蛋白免疫染色鉴定。
结果 接种24 h后细胞全部贴壁，48 h后人胸主动脉成纤维细胞体积增大并伸出伪足，形状以梭形或不规则形
为主；而大鼠胸主动脉的成纤维细胞生长的相对较慢。结论 用Ⅰ型胶原消化动脉的成纤维细胞效果较好，用
差速生长纯化的成纤维细胞可用于实验研究。

      【关键词】 胸主动脉；成纤维细胞；原代培养

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2012）06—023—03<

br>Primary culture of human and rat thoracic aortic fibroblast cells.
BAO Rong-hui, ZHOU Jun, ZHOU Chang, YAO
Zhi, LIAO Lei. Department of Ultrasound, the First College of Clinical Medical Science, China Three Gorges University (the
Central People's Hospital of Yichang City), Yichang 443003, Hubei, CHINA

【Abstract】 Objective To set up the method for the culture of fibroblasts from different sources. Methods
Human fibroblast cells were isolated through enzyme digestion and adherence, and then purified by differential attach-
ment technique. The morphology and phenotype characterization of cultured fibroblasts was inspected by light micros-
copy after HE and immunocytochemical staining for vimentin. Results 24 h after inoculation, all the cells had at-
tached. 48 h after inoculation, the volume of human artery fibroblast cell enlarged and pseudopodia stretched on, most-
ly in the shape of irregular or clostridial form. Rat thoracic aorta fibroblast cells grow relatively slower. Conclusion
In the primary culture of human and rat thoracic aortic fibroblast cells, it is recommended to digest by type-Ⅰ colla-
gen and purify by differential attachment technique.

      【Key words】 Thoracic aorta; Fibroblast cell; Primary culture
作者简介：鲍荣辉（1980—），女，湖北省宜昌市人，医师，硕士，研究方向：心血管超声诊断。
*通讯作者：周 军。E-mail：Zjsts8@163.com
·实验研究·