标题：NBR1、TTN蛋白表达水平与膀胱癌患者临床特征、预后的关系

      作者：萧超优，程章林，张岩    萧超优，程章林，张岩信阳市人民医院泌尿外科，河南 信阳 464000

      卷次： 2024年35卷14期

      【摘要】 目的 探讨自噬受体(NBR1)、肌联蛋白(TTN)水平与膀胱癌患者临床特征、预后的关系。方法 回顾性分析2018年6月至2020年5月信阳市人民医院收治的70例膀胱癌患者的临床资料，将患者的癌组织标本纳入膀胱癌组，对应癌旁组织标本纳入癌旁组。采用免疫组化法检测并比较两组标本组织的NBR1、TTN阳性表达率。分析NBR1、TTN表达与膀胱癌患者临床病理特征的关系，采用多因素Cox回归分析法探讨膀胱癌患者预后的影响因素。结果 膀胱癌组标本组织中的NBR1和 TTN阳性表达率分别为 74.29%、78.57%，明显高于癌旁组的40.00%、31.43%，差异均有统计学意义(P<0.05)；浸润深度T3~T4期、高分化、有淋巴结转移、TNM分期Ⅲ~Ⅳ期患者NBR1阳性表达率明显高于浸润深度T1~T2期、中/低分化、无淋巴结转移、TNM分期Ⅰ~Ⅱ期患者，差异均有统计学意义(P<0.05)；浸润深度T1~T2期、中/低分化、无淋巴结转移、TNM分期为Ⅰ~Ⅱ期、NBR1阴性、TTN阴性患者的3年生存率明显高于浸润深度T3~T4期、高分化、有淋巴结转移、TNM分期为Ⅲ~Ⅳ期、NBR1阳性、TTN阳性患者，差异均有统计学意义(P<0.05)。多因素Cox回归分析显示，高分化、TNM分期为Ⅲ~Ⅳ期、NBR1阳性、TTN阳性均是膀胱癌患者预后的影响因素(P<0.05)。结论 NBR1、TTN在膀胱癌中呈高表达，与浸润深度、TNM分期、分化程度、淋巴结转移密切相关，有望作为评估膀胱癌患者预后生物标志物。
      【关键词】 膀胱癌；自噬受体；肌联蛋白；临床病理；预后
      【中图分类号】 R737.14 【文献标识码】 A 【文章编号】 1003—6350（2024）14—1982—05

Relationship between expression levels of NBR1 and TTN proteins and clinical features and prognosis of patientswith bladder cancer.
XIAO Chao-you, CHENG Zhang-lin, ZHANG Yan. Department of Urology, Xinyang People'sHospital, Xinyang 464000, Henan, CHINA
【Abstract】 Objective To explore the relationship between the levels of autophagy receptor (NBR1) and titin(TTN) and the clinical features and prognosis of patients with bladder cancer. Methods The clinical data of 70 patientswith bladder cancer admitted to Xinyang People's Hospital from June 2018 to May 2020 were retrospectively analyzed.Cancer tissue specimens were included in the bladder cancer group, and corresponding paracancerous tissue specimenswere included in the paracancerous group. The expression levels of NBR1 and TTN were detected by immunohistochem-istry and then compared between the two groups. The relationship between the expression of NBR1 and TTN and theclinicopathological features of patients with bladder cancer was analyzed, and the prognostic factors of patients withbladder cancer were investigated by multivariate Cox regression analysis. Results The positive expression rates ofNBR1 and TTN in bladder cancer group were 74.29% and 78.57%, significantly higher than 40.00% and 31.43% in para-cancerous group (P<0.05). The positive expression rate of NBR1 in patients with infiltration depth of T3 to T4 stages,high differentiation, lymph node metastasis, and TNM stage Ⅲ to Ⅳ were significantly higher than those in patients withinfiltration depth of T1 to T2, medium or low differentiation, no lymph node metastasis, and TNM stage Ⅰ to Ⅱ (P<0.05). The 3-year survival rates of the patients with infiltration depth of T1 to T2 stage, medium or low differentiation, nolymph node metastasis, TNM stage Ⅰ to Ⅱ, NBR1 negative, and TTN negative were significantly higher thanthose in patients with infiltration depth of T3 to T4, high differentiation, lymph node metastasis, TNM stage Ⅲ toⅣ, NBR1 positive, TTN positive (all P<0.05). Multivariate Cox regression analysis showed that high differentia-tion, TNM stage Ⅲ to Ⅳ, NBR1 positive, and TTN positive were prognostic factors in patients with bladder cancer(P<0.05). Conclusion NBR1 and TTN are highly expressed in bladder cancer, which are closely related to thedepth of invasion, TNM stage, differentiation degree, and lymph node metastasis, and are expected to be used asbiomarkers to evaluate the prognosis of patients with bladder cancer.
      【Key words】 Bladder cancer; Autophagy receptor (NBR1); Titin (TTN); Clinicopathology; Prognosis