标题：迈瑞 BC-6800 Plus血小板相关参数参考区间的建立及验证

      作者：张宁 1，蒿花 2，孟欣 1    西安交通大学第一附属医院检验科 1、健康医学科 2，陕西 西安 710061

      卷次： 2024年35卷13期

      【摘要】 目的 建立并验证西安地区血小板相关参数血小板计数 (PLT)、血小板体积分布宽度标准差(PDW-SD)、平均血小板体积(MPV)、血小板压积(PCT)、大血小板比率(P-LCR)、大血小板数量(P-LCC)、血小板体积分布宽度(PDW)的参考区间。方法 选择2022年8月至2023年8月在西安交通大学第一附属医院主院区和东院区5 153名健康体检成年人群，采用迈瑞Mindray BC-6800 Plus血细胞分析仪检测外周血液，依据《临床实验室如何确定和建立生物参考区间；批准指南》CLSL C28-A3，以非参数法建立PLT、PDW-SD、MPV、PCT、P-LCR、P-LCC、PDW的参考区间。结果 健康成年人群男、女不同性别间的PLT、MPV、PCT、P-LCR、P-LCC、PDW比较差异均具有统计学意义(P<0.05)，但PDW-SD比较差异无统计学意义(P>0.05)；各年龄段PLT、PCT、P-LCC、PDW间比较差异均具有统计学意义(P<0.05)，但MPV、PDW-SD、P-LCR各年龄段间比较差异均无统计学意义(P>0.05)。标准正态离差检验方法显示无需按照性别、年龄段分组建立参考区间。PLT、MPV、PCT、PDW-SD、P-LCR、P-LCC、PDW的参考区间分别为(148~324)×109/L、8.4~12.5 fL、0.159%~0.322%、8.8~17.5 fL、15.1%~43.6%、(35~96)×109/L、15.1~16.8。结论 建立并验证西安地区血小板相关参数参考区间，合理选用PDW-SD对异常血小板的识别，对血小板参数的自动审核提供必要的数据支持。
      【关键词】 西安地区；血小板；血小板体积分布宽度；血细胞分析仪；参考区间
      【中图分类号】 R446.11 【文献标识码】 A 【文章编号】 1003—6350（2024）13—1895—06

Establishment and verification of reference intervals of platelet-related parameters in Mindray BC-6800 Plus.ZHANG Ning 1, HAO Hua 2, MENG Xin 1.
Department of Clinical Laboratory 1, Department of Health Medicine 2, the FirstAffiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shannxi, CHINA
【Abstract】 Objective To establish and verify the reference intervals of platelet count (PLT), platelet distribu-tion width-standard deviation (PDW-SD), mean platelet volume (MPV), plateletcrit (PCT), platelet-larger cell ratio(P-LCR), platelet-larger cell count (P-LCC), platelet distribution width (PDW) in Xi'an. Methods A total of 5 153healthy adults undergoing physical examination were selected in main campus and east campus of the First AffiliatedHospital of Xi'an Jiaotong University from August 2022 to August 2023. Mindray BC-6800 Plus hematology analyzerwas used to detect peripheral blood. According to the“How to determine and establish the biological reference interval”in clinical laboratory cLSL C28-A3, the reference intervals of PLT, PDW-SD, MPV, PCT, P-LCR, P-LCC, and PDWwere established by nonparametric method. Results There were statistically significant differences in PLT, MPV, PCT,P-LCR, P-LCC, and PDW between males and females (P<0.05), but no statistically significant differences were found inPDW-SD between males and females (P>0.05). There were statistically significant differences in PLT, PCT, P-LCC, andPDW among age groups (P<0.05); however, there was no significant difference in MPV, PDW-SD, and P-LCR amongage groups (P>0.05). The standard normal deviation test showed that there was no need to establish reference intervalsaccording to gender and age groups. The biological reference intervals of PLT, MPV, PCT, PDW-SD, P-LCR, P-LCCand PDW were 148×109/L to 324, 8.4-12.5 fL, 0.159-0.322%, 8.8-17.5 fL, 15.1-43.6%, 35×109/L to 96, 15.1-16.8.Conclusion The biological reference interval of platelet-related parameters in Xi'an was established and verified, andthe reasonable use of PDW-SD provided data support for the identification of abnormal platelets and auto verification ofplatelet parameters.
      【Key words】 Xi'an; Platelet (PLT); Platelet distribution width (PDW); Hematology analyzer; Reference intervals