标题：一株红树林真菌抗单纯疱疹病毒Ⅱ型的研究

      作者：裴 华，林英姿*，饶朗毓，王永霞，牛莉娜，王华民
    （海南医学院热带医学与检验医学院，海南 海口 571101）

      卷次： 2012年23卷3期

      【摘要】 目的 对分离自海南红树林真菌菌株 PH1018的胞外多糖（Exopolysaccharide，EPS）抗单纯疱疹
病毒Ⅱ型(Herpes simplex virus type 2，HSV-2)活性进行研究。方法 收集纯化菌株PH1018产生的EPS，体外采
用细胞病变观察EPS的细胞毒性和抗HSV-2作用。为观察EPS对HSV-2体内感染的治疗效果，小鼠颅内注射
0.02 ml滴度为 100 LD50/0.1 ml-1的HSV-2，24 h后以不同浓度EPS灌胃，持续 7 d，14 d后计算小鼠存活率和存活
时间。菌株分类采用形态学观察和 ITS序列分析。结果 EPS可抑制HSV-2病毒活性，病毒感染的细胞病变的
半抑制浓度(Inhibited concentration，IC50)为 313 μg/ml，SI为 11.43。EPS 25 mg·kg-1·d-1灌胃后，小鼠存活率为
40.0%，存活时间为（9.82±1.87）d，相比模型组实验动物存活时间和存活率均有提高。菌株初步鉴定为杂色曲
霉。结论 从海南红树林分离一株杂色曲霉，其产生的EPS具有抗HSV-2活性，值得进一步研究。

      【关键词】 真菌；红树林；胞外多糖；杂色曲霉；抗病毒作用；单纯疱疹病毒Ⅱ型

      【中图分类号】 R-322 【文献标识码】 A 【文章编号】 1003—6350（2012）03—001—03<

br>Study on antiviral activities of a fungi strain isolated from mangrove to herpes simplex virus type 2.
PEI Hua,
LIN Ying-zi*, RAO Lang-yu, WANG Yong-xia, NIU Li-na, WANG Hua-min. School of Tropical Medicine and Laboratory
Medicine, Hainan Medical College, Haikou 571101, Hainan, CHINA

【Abstract】 Objective To study the antiviral activities of the exopolysaccharide (EPS) of fungi strain
PH1018 against herpes simplex virus type 2 (HSV-2). Methods The EPS was collected from fungi strain PH1018, a
fungi strain isolated from mangrove. The antiviral activities of EPS were evaluated by observing the cytotoxicity and
cytopathic effect (CPE). Every mouse was injected intracerebrally with 0.02 ml HSV-2 solution with the titer of 100
LD50 0.1 ml-1. 24 hours later, the mice were treated by EPS with different doses for 7 days. The survival rate and surviv-
al time of mice was observed after 14 days. The taxonomic status of strain was identified by morphological observa-
tions and ITS sequence analysis. Results EPS inhibited HSV-2 at the IC50 value of 313 μg/ml and SI value of 11.43
in vitro. After treatment by EPS 25 mg.kg-1.d-1, the survival rate of the mice infected with HSV-2 was 40.0%. The aver-
age survival time was (9.82±1.87) days. The strain was identified as Aspergillus versicolor. Conclusion EPS from
strain PH1018 had remarkable anti HSV-2 activity, which worth for further research.

      【Key words】 Fungi; Mangrove; Exopolysaccharide; Aspergillus versicolor; Antiviral effect; Herpes simplex vi-
rus type 2
基金项目：国家自然科学基金项目(编号：30760001)，海南省自然科学基金资助项目(编号：琼科[2009]309033)；海南省自然科学基金资助
项目(编号：琼科[2009]309032)
作者简介：裴 华（1979—），男，安徽省舒城市人，讲师，硕士，研究方向：抗感染免疫。
*通讯作者：林英姿。E-mail:phzmh61@sohu.com .