标题：桑叶消渴胶囊对Ⅱ型糖尿病大鼠的影响

      作者：李 超，潘建明，罗新根，张相年，吴新荣*
    (广州军区广州总医院药剂科，广东 广州 510010)

      卷次： 2012年23卷2期

      【摘要】 目的 观察桑叶消渴胶囊对Ⅱ型糖尿病大鼠口服3 g/kg淀粉的糖耐量和连续给药29 d血糖、甘油
三酯的影响。方法 将SPF级Wistar大鼠用链尿佐菌素(STZ)造成Ⅱ型糖尿病模型，测定甘油三酯。按照血糖随
机分成空白对照组给予生理盐水、受试物低、高剂量组250 mg/kg、1 000 mg/kg，阳性对照二甲双胍200 mg/kg，糖
耐量实验阳性对照改为拜糖萍20 mg/kg，ig量按10.0 ml/kg BW计算。糖耐量实验当天动物禁食过夜测定空腹血
糖，给药时同时给予淀粉3 g/kg，于30 min、60 min、120 min后测血糖，并计算血糖升高值。降糖实验当天动物禁
食4 h后测定餐前血糖再给药，给药后240 min测定餐后血糖，尾静脉取血分离血清测定甘油三酯，计算各组给药
后较给药前血糖下降值及血糖下降百分率。结果 受试物低、高剂量组能抑制Ⅱ型糖尿病大鼠糖吸收，与空白
对照组比较 60 min、120 min差异有统计学意义(P<0.05，P<0.01)；连续给药 29 d能降低Ⅱ型糖尿病大鼠的血糖，
高剂量组与空白组比较差异有统计学意义(P<0.05)；低、高剂量组与空白组比较均能降低甘油三酯，差异有统计
学意义(P<0.05)。结论 桑叶消渴胶囊剂量达到250 mg/kg对Ⅱ型糖尿病大鼠口服淀粉糖耐量有显著的抑制糖
吸收作用，连续给药29 d剂量达到1 000 mg/kg能显著降低血糖和甘油三酯。

      【关键词】 桑叶消渴胶囊；Ⅱ型糖尿病；大鼠

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2012）02—018—03<

br>Effect of mulberry-leaf capsules on mice with typeⅡdiabetes.
LI Chao, PAN Jian-ming, LUO Xin-gen, ZHANG
Xiang-nian, WU Xin-rong. Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command
Area, Guangzhou 510010, Guangdong, CHINA

【Abstract】 Objective To observe the effect of Sangye Xiaoke Jiaonang (mulberry-leaf capsules) on the glu-
cose tolerance test (GTT) in mice with typeⅡ diabetes after oral intake of 3 g/kg amylum, and its effects on blood glu-
cose and triglycerides after being administrated for 30 consecutive days. Methods Wistar rats (SPF) model with
Type II diabetes was built by injection with streptozotocin (STZ). The blood and triglyceride was determined. The rats
were randomized into a control group (saline), low-dose group (250 mg/kg mulberry-leaf capsules), high-dose group
(1000 mg/kg mulberry-leaf capsules) and positive control group (200 mg/kg metformin). In GTT, the positive control
group is Glucobay (20 mg/kg). The dose of administration is determined by 10.0 ml/kg BW. The fasting glucose was
measured on the rats who had fasted over night on the day of GTT, The blood glucose was measured 30 min, 60 min,
120 min after intake of 3 g/kg amylum with mulberry-leaf capsules, with the growth of blood glucose level calculated.
The rats fasted for 4 h were measured for the pre-dinner blood glucose levels before administration, and then detected
for the post-dinner blood glucose levels and triglyceride levels 240 min after administration. The value and the rate of
decline in blood glucose were analyzed. Results Glucose absorption was inhibited in the low-dose group and
high-dose group, showing significant difference with that in the control group at 60 min and 120 min after administra-
tion (P<0.05, P<0.01). The blood glucose value in the model rats was declined after being administrated for 29 consec-
utive days. The difference between the high-dose group and the control group was statistically significant. The blood
and triglyceride level was declined in low-dose group and high-dose group, showing statistically significant difference
with the control group (P<0.05). Conclusion After being administrated mulberry-leaf capsules for 29 consecutive
days at the concentration of 1 000 mg/kg, significantly declined levels of blood glucose and triglyceride could be deter-
mined in rats with type II diabetes,

      【Key words】 Mulberry-leaf capsules; TypeⅡ Diabetes; Mice
基金项目：广东省科技计划资助项目(编号：2008A030201031)