标题：新冠核酸检测实验室环境消毒与监测结果分析

      作者：张德明，黄荣，梁琪，王静，韦唯，李树萍，孙翔，汪洪富，刘欣桐，黄婷婷，周义文    南方医科大学深圳医院检验科，广东 深圳 518000

      卷次： 2023年34卷17期

      【摘要】 目的 寻找新冠检测实验室核酸重点污染区域，探索排除新冠检测实验室污染的有效方法，提高新冠核酸检测结果的稳定性和准确性。方法 采用物体表面擦拭采样和空气沉降采样的方法对实验室高风险区域进行监测。监测点包括试剂准备区、标本制备区、扩增区、高压灭菌室等，每天进行物体表面擦拭和空气沉降采样监测，采样后的样本通过逆转录聚合酶链式反应(RT-PCR)判断是否存在核酸污染。根据检测结果对重点污染区域加强消毒，再通过RT-PCR监测消毒效果。结果 在监测时间内，实验室环境的靶基因(ORF1ab与N基因)监测结果均为阴性，显示各个监测点均无靶基因污染。而内标基因(RNase P基因)监测结果显示，物体表面擦拭法的阳性率分别为试剂准备区1.56%、标本制备区1.3%、办公室0.26%、标本转运箱0.26%；空气沉降法的阳性率分别为试剂准备区0.32%、标本制备区1.3%、扩增区0.16%。污染发生后，通过污染原因分析、清理消毒等措施，实验中监测到的污染区域样本均为阴性。结论 通过寻找新冠检测实验室核酸重点污染区域，对重点污染区域进行有效的环境消毒，可以及时有效地避免新冠检测实验室污染情况的发生，提高新冠核酸检测结果的稳定性和准确性。
      【关键词】 新型冠状病毒；核酸污染；环境消毒；环境监测
      【中图分类号】 R511 【文献标识码】 A 【文章编号】 1003—6350（2023）17—2544—05

Environmental disinfection and monitoring of COVID-19 nucleic acid testing laboratory.
ZHANG De-ming,HUANG Rong, LIANG Qi, WANG Jing, WEI Wei, LI Shu-ping, SUN Xiang, WANG Hong-fu, LIU Xin-tong, HUANGTing-ting, ZHOU Yi-wen. Department of Clinical Laboratory, Shenzhen Hospital of Southern Medical University, Shenzhen518000, Guangdong, CHINA
【Abstract】 Objective To identify the key areas of nucleic acid contamination in COVID-19 testing laboratories,explore effective methods for eliminating contamination, and improve the stability and accuracy of COVID-19 nucleic ac-id test results. Methods Surface swab and air sampling were used to monitor high-risk areas in the laboratory, includingthe reagent preparation area, specimen preparation area, amplification area, and high-pressure sterilization room. Surfaceswab and air sampling were conducted daily, and samples were tested using reverse transcription polymerase chain reac-tion (RT-PCR) to determine if there was nucleic acid contamination. Based on the test results, key areas of contaminationwere subjected to enhanced disinfection, and the disinfection effectiveness was monitored using RT-PCR. Results Dur-ing the monitoring period, the target genes (ORF1ab and N genes) in the laboratory environment were negative, indicatingthat there was no target gene contamination in any of the monitoring points. However, the internal reference gene (RNaseP gene) monitoring results showed that the positive rates of surface swabbing in the reagent preparation area, specimenpreparation area, office, and specimen transport box were 1.56%, 1.3%, 0.26%, and 0.26%, respectively, and the positiverates of air sampling in the reagent preparation area, specimen preparation area, and amplification area were 0.32%, 1.3%,and 0.16%, respectively. All the contaminated areas tested turned negative after analysis of the contamination sources anddisinfection measures. Conclusion By identifying key areas of nucleic acid contamination in COVID-19 testing labora-tories and performing effective environmental disinfection in those areas, laboratory contamination can be avoided in atimely and effective manner, and the stability and accuracy of COVID-19 nucleic acid test results can be improved.
      【Key words】 SARS-CoV-2; Nucleic acid contamination; Environmental disinfection; Environmental monitoring