标题：卡介苗提取物体外诱导小鼠脾淋巴细胞对人膀胱癌细胞的杀伤作用

      作者：蒋翡翎 1，单保恩 2，姚 敏 1，魏小斌 1，伍 燕 1，邓碧兰 1
    (1.海口市人民医院检验科，海南 海口 570208
2.河北医科大学第四医院科研中心，河北 石家庄 050011)

      卷次： 2012年23卷1期

      【摘要】 目的 分离活卡介苗(Bacille Calmette-Guérin，BCG)的有效组分，分析其有效组分诱导的小鼠脾淋
巴细胞体外对人膀胱癌细胞株T24细胞的杀伤作用。方法 采用酶裂解法和超声波破碎法对活BCG进行裂解，
通过Sephadex G100凝胶层析对其有效组分进行分离及分子量的测定，用SDS-PAGE凝胶电泳进行纯度鉴定；采
用四氮唑盐(MTT)法测定BCG有效组分BCGE2诱导的小鼠脾淋巴细胞对人膀胱癌细胞株T24细胞的杀伤活性。
结果 BCGE2可体外诱导小鼠脾淋巴细胞对膀胱癌细胞株T24细胞的杀伤作用，在效靶比为20:1时对T24细胞的
抑制率为42.31%，而对照组仅为13.32%，两者比较差异有统计学意义(P<0.01)。结论 BCGE2诱导的小鼠脾淋
巴细胞对人膀胱癌细胞株T24的杀伤作用很显著，且随着效靶比的增加而增强。

      【关键词】 BCG提取物；脾淋巴细胞；膀胱癌细胞株T24细胞；杀伤作用

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2012）01—018—03<

br>Killing effect of mouse spleen lymphocytes induced by active BCG extracts to human bladder cancer cell in vi-
tro.
JIANG Fei-ling 1, SHAN Bao-en 2, YAO Ming 1, WEI Xiao-bing 1, WU Yan 1, DENG Bi-lang 1. 1. Department of Clin-
ical Laboratory, Haikou Municipal People’s Hospital, Haikou 570208, Hainan, CHINA; 2. Research Center, the Fourth
Hospital of Hebei Medical University, Shijianzhuang 050011, Hebei, CHINA

【Abstract】 Objective To extract, isolate, purify and identify the active components of Bacille Calmette-Guérin
(BCG), and to analyze the killing effect of mouse spleen lymphocytes induced by active BCG extracts to human blad-
der cancer cell line T24 in vitro. Methods Live BCG was divided by the enzyme and ultrasonic method to extract the
active protein. The components of BCG extracts was isolated, and analyzed for the molecular weight by Sephadex gel
filtration chromatography, then further studied to identify the purity and the molecular weight by the SDS-PAGE gela-
tin electrophoresis. The killing activity of mouse spleen lymphocytes induced by the active component BCGE2 to the
human bladder cancer cell line T24 was analyzed by MTT method. Results The mouse spleen lymphocytes induced
by BCGE2 could kill the bladder cancer cell line T24 in vitro. When the effect cells to the target cells was 20:1, the inhib-
it rate was 42.31% , which was significantly higher than 13.32% in the control group (P<0.01). Conclusion The
mouse spleen lymphocytes induced by BCGE2 have obvious killing effect on the bladder cancer cell T24 in dose-depen-
dent manner.

      【Key words】 BCG extracts; Spleen lymphocytes; Bladder cancer cell T24; Killing effect
作者简介：蒋翡翎(1973—)，女，海南省乐东市人，副主任技师，本科。
·实验研究·
稳定性碘对大鼠吸 131I率及有效半衰期的影响
高光东，肖 欢，李诗运，姚爱珠，白殿卿
（海南省人民医院核医学科，海南 海口 570311）