标题：基于网络药理学的淫羊藿治疗糖尿病心肌病的机制研究

      作者：梁金花 1，林俊颜 2，李春河 3，罗苑苑 3，陈日垦 2，郑珍珍 4    1.广东医科大学附属第二医院内分泌科，广东 湛江 524000；2.国家呼吸医学中心、呼吸疾病国家重点实验室、广州医科大学附属第一医院呼吸与危重症医学科、广州呼吸健康研究院，广东 广州 510120；3.广州中医药大学第一附属医院重症医学科，广东 广州 510120；4.广东医科大学附属第二医院呼吸科，广东 湛江 524000

      卷次： 2023年34卷1期

      【摘要】 目的 研究显示淫羊藿及其有效成分在心血管疾病中有很好的保护作用，但在糖尿病心肌病(DCM)中仍未有研究，因此本研究以网络药理学为基础，从作用机制角度针对DCM受到淫羊藿治疗的影响进行研究。方法 结合研究的需求由TCMSP、PubChem和Swiss Target Prediction数据库获取淫羊藿(yinyanghuo)的主要有效活性成分和靶点信息，基于GeneCards数据库获取糖尿病心肌病(DCM)相关靶点，对靶点网络进行构建时选择Cytoscape 3.8.2工具，同时还应用PPI网络,选择R语言工具进行KEGG与GO富集分析。结果 共获得17种主要有效活性成分和58个淫羊藿与DCM相关靶点，PPI网络中核心基因为AKT1、TNF、VEGFA和TP53等；GO功能富集后富集数目较多的有 protein serine/threonine kinase activity、insulin receptor substrate binding、proteinkinase C activity、calcium-dependent protein kinase C activity、RNA polymerase Ⅱ-specific DNA-binding transcriptionfactor binding和DNA-binding transcription factor binding等；KEGG富集通路分析显示淫羊藿治疗糖尿病心肌病主要通路有Diabetic cardiomyopathy、AGE-RAGE signaling pathway in diabetic complications Insulin resistance、HIF-1signaling pathway、Hepatitis B和Proteoglycans in cancer等；KEGG通路与基因之间的网络图中关联数量较多的基因主要有AKT1、PIK3CB、PIK3CA、PIK3R1和PIK3CD等。结论 淫羊藿可能利用多靶点、多成分、多通路抑制氧化应激、炎症反应、细胞自噬来改善DCM及心肌细胞氧化代谢功能。
      【关键词】 糖尿病心肌病；淫羊藿；网络药理学；靶点；作用机制
      【中图分类号】 R587.2 【文献标识码】 A 【文章编号】 1003—6350（2023）01—0091—09

Mechanism of Epimedium in treatment of diabetic cardiomyopathy based on network pharmacology.
LIANGJin-hua 1, LIN Jun-yan 2, LI Chun-he 3, LUO Yuan-yuan 3, CHEN Ri-ken 2, ZHENG Zhen-zhen 4. 1. Department ofEndocrinology, the Second Affiliated Hospital of Guangdong Medical University, Zhangjiang 524000, Guangdong, CHINA;2. National Clinical Research Center for Respiratory Disease/State Key Laboratory of Respiratory Disease/Department ofRespiratory and Critical Care Medicine, the First Affiliated Hospital of Guangzhou Medical University/Guangzhou Institute ofRespiratory Disease, Guangzhou 510120, Guangdong, CHINA; 3. Department of Critical Care Medicine, GuangzhouUniversity of Chinese Medicine, Guangzhou 510120, Guangdong, CHINA; 4. Department of Respiration, the Second AffiliatedHospital of Guangdong Medical University, Zhangjiang 524000, Guangdong, CHINA
【Abstract】 Objective Studies have shown that Epimedium (Yinyanghuo) and its active ingredients have agood protective effect on cardiovascular disease, but it has not been studied in diabetic cardiomyopathy (DCM). There-fore, this study, based on network pharmacology, investigated the effect of Epimedium treatment on DCM from the per-spective of mechanism of action. Methods The main active ingredients and target information of Epimedium (yinyang-huo) were obtained from TCMSP, PubChem, and Swiss Target Prediction database in combination with the needs of re-search. Targets related to diabetic cardiomyopathy (DCM) were obtained based on the GeneCards database. The Cyto-scape 3.8.2 tool was selected to construct the target network, the PPI network was also applied, and the R language toolwas selected for KEGG and GO enrichment analysis. Results A total of 17 main active ingredients and 58 Epimediumand DCM related targets were obtained. The core genes in the PPI network were AKT1, TNF, VEGFA, and TP53. AfterGO function enrichment, the enriched numbers were protein serine/threonine kinase activity, insulin receptor substrate