标题：MARCH5对卵巢癌细胞糖代谢的调控作用研究

      作者：杜娟 1，何莉茹 1，王艳艳 1，王粉玲 1，金大永 2    1.西安市北方医院妇科，陕西 西安 710043；2.西安市大兴医院影像科，陕西 西安 710016

      卷次： 2022年33卷18期

      【摘要】 目的 探讨膜相关锌指蛋白 5 (MARCH5)在卵巢癌细胞糖代谢中的调控作用。方法 将卵巢癌SKOV3细胞分为对照组(siCtrl)、MARCH5干涉组1 (siMARCH5#1)与MARCH5干涉组2 (siMARCH5#2)，分别用葡萄糖摄取、乳酸生成、氧耗速率、ATP产生及代谢组学实验，分析下调MARCH5对卵巢癌细胞糖代谢的影响。结果 与对照组SKOV3细胞相比，MARCH5干涉组1与干涉组2细胞的葡萄糖摄取[(1.00±0.06) vs (0.45±0.02) vs (0.44±0.02)]与乳酸产生[(1.00±0.05) vs (0.36±0.02) vs (0.35±0.03)]显著减少，而氧耗速率[(3.15±0.10) vs (5.73±0.14) vs (5.67±0.11)]与ATP含量[(1.00±0.04) vs (1.69±0.06) vs (1.74±0.07)]则显著升高，差异均有统计学意义(P<0.05)；此外，与对照组SKOV3细胞相比，MARCH5干涉组1与干涉组2细胞内糖酵解代谢物显著减少，而线粒体三羧酸循环代谢物显著增多，差异均有统计学意义(P<0.05)。结论 MARCH5促进了卵巢癌细胞糖代谢由氧化磷酸化向糖酵解的转变。
      【关键词】 卵巢癌；膜相关锌指蛋白5；E3泛素连接酶；糖酵解；氧化磷酸；调控作用
      【中图分类号】 R737.31 【文献标识码】 A 【文章编号】 1003—6350（2022）18—2313—05

Effect of MARCH5 on glucose metabolism in ovarian cancer cells.
DU Juan 1, HE Li-ru 1, WANG Yan-yan 1,WANG Fen-ling 1, JIN Da-yong 2. 1. Department of Gynaecology, Xi'an North Hospital, Xi'an 710043, Shaanxi, CHINA;2. Department of Medical Imaging, Xi'an Daxing Hospital, Xi'an 710016, Shaanxi, CHINA
【Abstract】 Objective To explore the role of membrane-associated RING-CH 5 (MARCH5) in the regulationof glucose metabolism in ovarian cancer cells. Methods Glucose uptake, lactate production, oxygen consumption rate,ATP production, and cell metabolomics were analyzed in SKOV3 cells without (siCtrl group) or with (siMARCH5#1group and siMARCH5#2 group) MARCH5 knockdown. Results Decreased glucose uptake [(1.00 ± 0.06) in siCtrlgroup vs (0.45±0.02) in siMARCH5#1 group vs (0.44±0.02) in siMARCH5#2 group] and decreased lactate production[(1.00±0.05) vs (0.36±0.02) vs (0.35±0.03)], whereas increased oxygen consumption rate [(3.15±0.10) vs (5.73±0.14) vs(5.67±0.11)] and increased ATP production [(1.00±0.04) vs (1.69±0.06) vs (1.74±0.07)] were observed in SKOV3 cellswhen MARCH5 was knocked-down. Reduced metabolites in glycolysis and increased metabolites in TCA cycle were ob-served in SKOV3 cells when MARCH5 was knocked-down. Conclusion MARCH5 promotes glucose metabolism re-programming in ovarian cancer cells through increasing glycolysis and decreasing oxidative phosphorylation.
      【Key words】 Ovarian cancer; MARCH5; E3 ubiquitin ligases; Glycolysis; Oxidative phosphorylation; Regulation