标题：硝酸镓和多黏菌素B抑制耐碳青霉烯类鲍曼不动杆菌生长的作用

      作者：王浩平 1，郑淑华 2，穆小萍 2，黄瑞玉 2，卢春敏 2
    (1.广东省人民政府机关门诊部，广东 广州 510030；
2.广东省妇幼保健院检验科，广东 广州 511400)

      卷次： 2016年27卷1期

      【摘要】 目的 评价硝酸镓和多黏菌素B对临床分离的耐碳青霉烯类鲍曼不动杆菌生长的抑制作用，分析其抗
菌机制。方法 采用微量稀释法检测硝酸镓和多黏菌素B对20株耐碳青霉烯类鲍曼不动杆菌的抑制作用，观察两者
联合应用的抑菌情况。结果 试验菌株对硝酸镓和多黏菌素B均敏感，硝酸镓对试验菌株的最小抑菌浓度(MIC)均为
16 μmol/L，多黏菌素B对试验菌株的MIC均为≤2.0 μg/ml，两者联合应用对耐碳青霉烯类鲍曼不动杆菌生长有协
同抑菌作用。结论 硝酸镓和多黏菌素B对耐碳青霉烯类鲍曼不动杆菌有明显抑菌效果，有望成为控制其蔓延感
染有效的治疗药物，为临床治疗这类难治细菌感染提供药物选择。

      【关键词】 硝酸镓；多黏菌素B；碳青霉烯类耐药鲍曼不动杆菌；抑制作用

      【中图分类号】 R378 【文献标识码】 A 【文章编号】 1003—6350（2016）01—0073—03


Inhibitory effect of gallium nitrate and polymyxin B on the growth of carbapenem-resistant Acinetobacter
baumannii.
WANG Hao-ping 1, ZHENG Shu-hua 2, MU Xiao-ping 2, HUANG Rui-yu 2, LU Chun-min 2. 1. Outpatient
Department, Guangdong Provincial People's Government, Guangzhou 510030, Guangdong, CHINA; 2. Department of
Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou 511400, Guangdong, CHINA

【Abstract】 Objective To investigate the ability of gallium nitrate and polymyxin B for inhibiting the growth of
carbapenem-resistant Acinetobacter baumannii isolated from patients, and to analyze their mechanisms. Methods The
inhibitory activity of gallium nitrate and polymyxin B against 20 carbapenem-resistant Acinetobacter baumannii strains
was tested by using microtiter plates. The activity of the combination of gallium nitrate and polymyxin B was also ob-
served. Results The tested strains were sensitive to gallium nitrate and polymyxin B. The minimum inhibitory concen-
tration (MIC) of gallium nitrate was 16 μmol/L, and the MIC of polymyxin B was less than 2.0 μg/ml. Synergistic inhibi-
tory effect for carbapenem-resistant Acinetobacter baumannii was observed when gallium nitrate and polymyxin B were
applied in combination. Conclusion The bacteriostasis of gallium nitrate and polymyxin B for carbapenem-resistant
Acinetobacter baumannii was obvious. It could be used for controlling the infection of carbapenem-resistant Acinetobacter
baumannii.

      【Key words】 Gallium nitrate; Polymyxin B; Carbapenem-resistant Acinetobacter baumannii; Inhibitory effect
·论 著·
6350.2016.01.024