标题：变应性鼻炎患者外周血PBMC中miR-146a和STAT5b的表达及临床意义

      作者：王忠勋，杨超，曹永华    陕西中医药大学第二附属医院耳鼻喉科，陕西 西安 712000

      卷次： 2022年33卷7期

      【摘要】 目的 探讨变应性鼻炎患者外周血单个核细胞(PBMC)中微小RNA-146a (miR-146a)、信号转导和转录激活因子5b (STAT5b)的表达及其临床意义。方法 选取 2017年1月至2020年12月在陕西中医药大学第二附属医院治疗的87例变应性鼻炎患者纳入观察组(其中轻度39例，中重度48例)，同时选取在我院治疗的鼻中隔偏曲患者 80例作为对照组，检测并比较两组患者PBMC中的miR-146a和STAT5b表达水平，同时采用Pearson法分析miR-146a、STAT5b相对表达量与鼻部症状评分相关性。结果 观察组患者PBMC中miR-146a和STAT5b相对表达量分别为2.15±0.98和0.58±0.18，明显低于对照组的3.65±1.00和1.53±0.34，差异均具有统计学意义(P<0.05)；观察组中重度患者 PBMC中miR-146a和 STAT5b相对表达量分别为 1.94±0.92和 0.41±0.16，明显低于轻度患者的2.41±0.97和0.79±0.20，差异均有统计学意义(P<0.05)；观察组患者PBMC中miR-146a和STAT5b相对表达量与鼻部症状评分呈负相关(r=-0.544、-0.577，P<0.05)，miR-146a与STAT5b呈正相关(r=0.645，P<0.05)。结论 变应性鼻炎患者PBMC中miR-146a和STAT5b表达下调，与患者病情程度呈负相关，同时两者间存在正相关。
      【关键词】 变应性鼻炎；单个核细胞；微小RNA-146a；信号转导和转录激活因子5b；病情程度
      【中图分类号】 R765.21 【文献标识码】 A 【文章编号】 1003—6350（2022）07—0879—04

Expression and clinical significance of miR-146a and STAT5b in peripheral blood mononuclear cells of patientswith allergic rhinitis.
WANG Zhong-xun, YANG Chao, CAO Yong-hua. Department of Otolaryngology, the SecondAffiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xi'an 712000, Shaanxi, CHINA
【Abstract】 Objective To investigate the expression and clinical significance of microRNA-146a (miR-146a),signal transducer and activator of transcription 5b (STAT5b) in peripheral blood mononuclear cells (PBMC) of patientswith allergic rhinitis. Methods A total of 87 patients with allergic rhinitis treated in the Second Affiliated Hospital ofShaanxi University of Traditional Chinese Medicine from January 2017 to December 2020 were selected as the observa-tion group (39 cases were mild and 48 cases were moderate and severe), and 80 patients with deviated nasal septum wereselected as the control group. The expression levels of miR-146 a and STAT5b in PBMC were detected and comparedbetween the two groups. Pearson method was used to analyze the correlation between the relative expression levels ofmiR-146 a and STAT5 b and nasal symptom score. Results The relative expression levels of miR-146a and STAT5b inPBMC of the observation group were 2.15±0.98 and 0.58±0.18, respectively, which were significantly lower than 3.65±1.00 and 1.53±0.34 of control group (P<0.05). The relative expression levels of miR-146a and STAT5b in PBMC of pa-tients with moderate to severe degree in the observation group were 1.94±0.92 and 0.41±0.16, respectively, which weresignificantly lower than 2.41±0.97 and 0.79±0.20 in patients with mild degree (P<0.05). The relative expression levels ofmiR-146a and STAT5b in PBMC of the observation group were negatively correlated with nasal symptom score(r=-0.544, -0.577, P<0.05), and miR-146a was positively correlated with STAT5b (r=0.645, P<0.05). Conclusion Theexpression of miR-146a and STAT5b in PBMC of patients with allergic rhinitis was down-regulated, which was nega-tively correlated with the severity of the disease, and there was a positive correlation between them.
      【Key words】 Allergic rhinitis; Mononuclear cells; microRNA-146a; Signal transducer and activator of transcrip-tion 5b; Degree of illness