标题：降钙素基因相关肽对重症中暑大鼠脑细胞凋亡的作用研究

      作者：邱婷 1，刘志峰 2，陆程翔 3    1.厦门大学附属中山医院神经内科，福建 厦门 361004；2.南部战区总医院重症医学科，广东 广州 510010；3.厦门大学附属中山医院重症医学科，福建 厦门 361004

      卷次： 2022年33卷1期

      【摘要】 目的 探讨降钙素基因相关肽(CGRP)对重症中暑大鼠脑细胞凋亡的影响。方法 应用可调节人工气候舱建立大鼠重症中暑模型。将20只大鼠分为对照组、热打击组(HS组)、热打击+注射CGRP组(HS+CGRP组)、热打击+注射CGRP8-37组(HS+CGRP8-37组)，每组 5只。在热打击后HS+CGRP组大鼠均立即经颈动脉注射CGRP，HS+CGRP8-37组大鼠均立即经颈动脉注射CGRP8-37。所有大鼠于造模2 h后取脑组织检测细胞凋亡、半胱天冬酶-3 (Caspase-3)、B淋巴细胞瘤-2基因蛋白(Bcl-2)。结果 与HS组大鼠比较，HS+CGRP组大鼠的脑细胞凋亡水平明显减少，而HS+CGRP8-37组大鼠则显著增加，差异均有统计学意义(P<0.05)，而与对照组比较，HS组大鼠脑细胞凋亡水平有显著升高，差异有统计学意义(P<0.05)；与HS组大鼠比较，HS+CGRP组大鼠的脑组织活化Caspase-3蛋白水平明显减少，而HS+CGRP8-37组则显著增加，差异均有统计学意义(P<0.05)；HS组大鼠的Bcl-2蛋白表达水平较对照组明显下降，注射CGRP后，Bcl-2蛋白水平较HS组显著增加，差异均有统计学意义(P<0.05)；注射CGRP8-37后，Bcl-2显著下降，与对照组比较差异有统计学意义(P<0.05)。结论 CGRP对重症中暑大鼠脑细胞凋亡有抑制作用。
      【关键词】 雄性Wistar大鼠；重症中暑；降钙素基因相关肽；脑；凋亡；半胱天冬酶-3；B淋巴细胞瘤-2基因蛋白
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2022）01—0001—04

Study on the effect of calcitonin gene-related peptide on brain cells apoptosis in rats with heatstroke.
QIU Ting 1,LIU Zhi-feng 2, LU Cheng-xiang 3. 1. Department of Neurology, Zhongshan Hospital Xiamen University, Xiamen 361004,Fujian, CHINA; 2. Department of Critical Medicine, PLA Southern Theater Command General Hospital, Guangzhou 510010,Guangdong, CHINA; 3. Department of Critical Medicine, Zhongshan Hospital Xiamen University, Xiamen 361004, Fujian,CHINA
【Abstract】 Objective To investigate the effect of calcitonin gene-related peptide (CGRP) on brain cells apopto-sis in rats with heatstroke. Methods The model of heatstroke in rats was established by using an adjustable artificial cli-mate chamber. Twenty rats were divided into control group, heat stroke group (HS group), heat stroke and CGRP injec-tion group (HS+CGRP group), heat stroke and CGRP8-37 injection group (HS+CGRP8-37 group), with 5 rats in eachgroup. After the heat shock, rats in the HS+CGRP group were immediately injected with CGRP through the carotid ar-tery, and rats in the HS+CGRP8-37 group were immediately injected with CGRP8-37 through the carotid artery. Thebrain tissues of all rats were taken 2 h after modeling to detect the level of cell apoptosis, Caspase-3, and B-cell lympho-ma 2 (Bcl-2) protein. Results Compared with the HS group, the brain cell apoptosis level of the HS+CGRP group wassignificantly decreased, while that of HS + CGRP8-37 group was significantly increased (P<0.05); compared with thecontrol group, the brain cell apoptosis level of the HS group was significantly increased (P<0.05). Compared with theHS group, the expression of activated Caspase-3 protein of the HS+CGRP group was significantly decreased (P<0.05),while that of the HS+CGRP8-37 group was significantly increased (P<0.05). The expression level of Bcl-2 protein inthe HS group was significantly lower than that in the control group; however, after injection of CGRP, the Bcl-2 expres-sion level increased significantly compared with the HS group (P<0.05). After injection of CGRP8-37, the Bcl-2 expres-sion level decreased significantly compared with the control group (P<0.05). Conclusion CGRP can inhibit the apopto-sis of brain cells in rats with heatstroke.
      【Key words】 Male Wistar rats; Heatstroke; Calcitonin gene-related peptide (CGRP); Brain; Apoptosis; Cas-pase-3; Bcl-2 protein