标题：183株沙门菌属细菌的血清型分布、分子分型特征及耐药性分析

      作者：石红，王毅    宝鸡市中心医院检验科，陕西 宝鸡 721008

      卷次： 2021年32卷18期

      【摘要】 目的 分析沙门菌属细菌的血清型分布、分子分型特征及耐药性。方法 183株沙门菌属细菌来自宝鸡市中心医院2016年2月至2020年10月门诊及住院患者的临床送检标本，分析沙门菌属细菌的血清型分布、分子分型特征和耐药性情况。结果 183株沙门菌属细菌中鼠伤寒沙门菌占65.03%，为最常见血清型，其次为肠炎沙门菌 26.78%，副伤寒沙门菌、伤寒沙门菌、甲型副伤寒沙门菌、斯坦利沙门菌、德尔卑沙门菌分别占 1.64%、1.64%、1.09%、1.09%、1.09%；119株鼠伤寒沙门菌可见81个脉冲场凝胶电泳(PFGE)型，29株肠炎沙门菌可见21个PFGE型；沙门菌属细菌对四环素、头孢哌酮、链霉素、萘啶酸、氨苄西林的耐药率较高，耐药率分别为 89.62%、73.77%、78.14%、85.79%、88.52%；对卡那霉素、头孢他啶、头孢吡肟、头孢噻肟、头孢曲松、阿莫西林/克拉维素的耐药率较低，耐药率分别为 15.85%、10.93%、8.74%、18.03%、13.66%、12.57%；对环丙沙星、头孢西丁、亚胺培南均敏感，敏感率均为100%。结论 沙门菌属细菌的血清型分布和PFGE分型较多，且对多种抗生素耐药，临床应结合药敏试验结果，指导合理用药。
      【关键词】 沙门菌属细菌；鼠伤寒沙门菌；肠炎沙门菌；血清型；分子分型；耐药性
      【中图分类号】 R378.2+2 【文献标识码】 A 【文章编号】 1003—6350（2021）18—2392—03

Serotype distribution, molecular typing characteristics, and drug resistance analysis of 183 strains of Salmonella.SHI Hong, WANG Yi.
Department of Clinical Laboratory, Baoji Central Hospital, Baoji 721008, Shaanxi, CHINA
【Abstract】 Objective To analyze the serotype distribution, molecular typing characteristics, and drug resis-tance of Salmonella. Methods A total of 183 strains of Salmonella bacteria were collected from clinical samples of out-patients and inpatients in Baoji Central Hospital from February 2016 to October 2020. The serotype distribution, molecu-lar typing characteristics, and drug resistance of Salmonella bacteria were analyzed. Results Among 183 strains of Sal-monella, Salmonella typhimurium (65.03%) was the most common serotype, followed by Salmonella enteritidis (26.78%),and then Salmonella paratyphi (1.64%), Salmonella typhi (1.64%), Salmonella paratyphi A (1.09%), Salmonella stanley(1.09%), and Salmonella Delbert (1.09%), respectively. There were 81 pulsed field gel electrophoresis (PFGE) types in119 Salmonella typhimurium strains and 21 PFGE types in 29 Salmonella enteritidis strains. Salmonella showed high re-sistance to tetracycline, cefoperazone, streptomycin, nalidixic acid, and ampicillin (89.62%, 73.77%, 78.14%, 85.79%,and 88.52%, respectively), low resistance to kanamycin, ceftazidime, cefepime, cefotaxime, ceftriaxone, and amoxicil-lin/clavulanate (15.85%, 10.93%, 8.74%, 18.03%, 13.66%, and 12.57%, respectively, and were sensitive to ciprofloxa-cin, cefoxitin and imipenem (the sensitivity rate was 100%). Conclusion Salmonella has many serotypes and PFGEtypes, and is resistant to many antibiotics. The clinical results of drug sensitivity test should be used to guide rationaldrug use.
      【Key words】 Salmonella bacteria; Salmonella typhimurium; Salmonella enteritidis; Serotype; Molecular typing;Drug resistance·短篇论著·doi:10.3969/j.issn.1003-6350.2021.18.022