标题：基于原代细胞培养的三维肿瘤药筛模型的初步研究

      作者：王健 1，2，3，张荟蓉 2，3，4，5，赵盼 2，3，4，5，王光锁 1，2，3，邹畅 2，3，4，5    1.深圳市人民医院胸外科，广东 深圳 518000；2.暨南大学第二临床医学院，广东 深圳 518000；3.南方科技大学第一附属医院，广东 深圳 518052；4.深圳市人民医院临床医学研究中心，广东 深圳 518000；5.深圳市肿瘤精准医疗与分子诊断公共服务平台，广东 深圳 518000

      卷次： 2021年32卷14期

      【摘要】 目的 以原代培养的肺癌肿瘤细胞为基础，建立一种体外肿瘤药筛模型。方法 通过将分离得到的肿瘤细胞与饲养层细胞共培养，使肿瘤细胞在体外得到初步扩增。培养得到的肿瘤细胞与海藻酸钠溶液混合后滴进氯化钙溶液中形成稳定的海藻酸钙凝胶微球(水凝胶)，凝胶微球为细胞提供一个模拟的三维生长环境，通过传统的CCK8细胞毒性实验进行药物筛选。结果 基于原代细胞培养建立的三维模型中，形成的海藻酸钙凝胶微球大小均一，形态完整，该凝胶微球可用CCK8细胞毒性实验进行药物筛选，细胞毒性实验提示顺铂、卡铂及盐酸吉西他滨对该原代细胞的抑制率相对较高。结论 海藻酸钙凝胶微球三维体系的形成表明基于原代培养的三维肿瘤模型已初步建立，并且该模型可用作药物筛选，为临床用药提供一定的指导。
      【关键词】 原代肺癌细胞；共培养；三维培养；海藻酸钠；氯化钙；药物筛选模型
      【中图分类号】 R73-3 【文献标识码】 A 【文章编号】 1003—6350（2021）14—1778—04

Preliminary test of a three-dimensional tumor drug screening model based on primary cell culture.
WANG Jian 1, 2, 3,ZHANG Hui-rong 2, 3, 4, 5, ZHAO Pan 2, 3, 4, 5, WANG Guang-suo 1, 2, 3, ZOU Chang 2, 3, 4, 5. 1. Department of Thoracic Surgery,Shenzhen People's Hospital, Shenzhen 518000, Guangdong, CHINA; 2. The Second Clinical Medical College of JinanUniversity, Shenzhen 518000, Guangdong, CHINA; 3. The First Affiliated Hospital of Southern University, Shenzhen 518052,Guangdong, CHINA; 4. Clinical Medical Research Center, Shenzhen People's Hospital, Shenzhen 518000, Guangdong,CHINA; 5. Shenzhen Public Service Platform on Tumor Precision Medicine and Molecular Diagnosis, Shenzhen 518000,Guangdong, CHINA
【Abstract】 Objective To establish a model for tumor drug screening in vitro based on primary lung cancer cellculture. Methods By co-culturing the isolated tumor cells with feeder cells, the tumor cell were amplified in vitrofirstly. The cultured tumor cells were mixed with sodium alginate solution and then dropped into calcium chloride solu-tion to form stable calcium alginate gel microspheres (Hydrogels), which provided a three-dimensional growth environ-ment for the cells and could be used for drug screening by traditional CCK8 cytotoxicity test. Results In the three-di-mensional model based on the primary cell culture, calcium alginate gel microspheres were uniform in size and completein shape, and these gel microspheres can be used for drug screening in CCK8 cytotoxicity test. The cytotoxicity test indi-cated that cisplatin, carboplatin and gemcitabine hydrochloride had a relatively high inhibited rate to the primary cells.Conclution The formation of calcium alginate gel indicated a three-dimensional model based on primary cell culture waspreliminarily established, which also could be used as a drug screening model to provide guidance for clinical medication.
      【Key words】 Primary lung cell; Co-culture; Three-dimensional culture; Sodium alginate; Calcium chloride;Drug-screening model