标题：丙肝病毒感染对C反应蛋白表达的影响

      作者：袁丽 1，刘芳 2，邬开朗 2，李一荣 1
    (1.武汉大学中南医院检验科，湖北 武汉 430071；
2.武汉大学生命科学学院，湖北 武汉 430072)

      卷次： 2016年27卷18期

      【摘要】 目的 探讨丙型肝炎病毒(HCV)在体内外对C反应蛋白(CRP)表达的影响。方法 收集武汉大学中
南医院2015年1月至2016年2月HCV感染患者标本106例和健康体检者标本110例，免疫透射比浊法检测其血清
CRP水平，采用逆转录聚合酶链反应(RT-PCR)检测HCV感染的Huh7.5.1细胞及其对照细胞中CRP mRNA的表
达。结果 HCV感染患者血清CRP水平为(8.62±5.56) mg/L，显著高于健康体检者的(1.35±0.71) mg/L，差异有显著
统计学意义(P<0.01)；HCV感染的Huh7.5.1细胞中CRP mRNA的表达水平较对照细胞高，其CRP/β-actin灰度比值
分别为0.31和1.5。结论 HCV感染能够上调肝细胞CRP的表达。

      【关键词】 丙型肝炎病毒；C反应蛋白；逆转录聚合酶链反应；表达

      【中图分类号】 R512.6+3 【文献标识码】 A 【文章编号】 1003—6350（2016）18—2932—02


Effect of hepatitis C virus infection on the expression of C-reactive protein.
YUAN Li 1, LIU Fang 2, WU Kai-lang 2,
LI Yi-rong 1. 1. Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan 430071, Hubei, CHINA;
2. College of Life Sciences of Wuhan University, Wuhan 430072, Hubei, CHINA

【Abstract】 Objective To investigate the effect of hepatitis C virus (HCV) on the expression of C-reactive pro-
tein (CRP) in vitro and in vivo. Methods A total of 106 samples of HCV infected patients and 110 samples of healthy in-
dividuals in Zhongnan Hospital of Wuhan University from January 2015 to February 2016 were collected. Serum CRP
levels were measured by immune transmission turbidity method. The expression of CRP mRNA in Huh7.5.1 cells infect-
ed with HCV and control cells was measured by reverse transcription polymerase chain reaction (RT-PCR). Results Se-
rum CRP levels were (8.62±5.56) mg/L in patients with HCV infection, which were significantly higher than those in
healthy individuals (1.35 ± 0.71) mg/L, with statistically significant difference (P<0.01). The expression level of CRP
mRNA in Huh7.5.1 cells infected with HCV was significantly higher compared with that in control cells, and the gray ra-
tio of CRP/ beta-actin was 0.31 and 1.5 respectively. Conclusion HCV infection can upregulate the expression of CRP
in liver cells.

      【Key words】 Hepatitis C virus; C-reactive protein; Reverse transcription polymerase chain reaction; Expression
·论 著·
6350.2016.18.003