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标题:乳腺癌组织中miR218-5p、IGF-1的表达水平及其临床意义 作者:黄方杰,黄波,张艺超,许酼敏 卷次: 2021年32卷11期
中国科学院大学深圳医院(光明)甲乳外科,广东 深圳 518106【摘要】 目的 探讨乳腺癌组织中微小 RNA-218-5p (miR218-5p)、胰岛素样生长因子-1 (IGF-1)表达水平的变化及其临床意义。方法 选取中国科学院大学深圳医院(光明)甲乳外科2016年6月至2019年8月经手术切除的120例乳腺癌组织蜡块标本作为乳腺癌组,另随机抽取60例乳腺癌患者标本切缘的癌旁正常组织(距癌旁组织的距离超过5 cm)作为癌旁组,并选择我院同期住院治疗的90例良性乳腺纤维瘤病变组织作为良性病变组。比较三组组织标本miR218-5p、IGF-1水平的表达,比较乳腺癌组不同TNM分期患者组织标本miR218-5p、IGF-1水平的表达,采用Spearman相关性分析法分析miR218-5p、IGF-1与TNM分期的相关性。结果 癌旁组组织中miR218-5p、IGF-1的阳性细胞表达率分别为(1.21±0.28)%、(2.63±0.20)%,良性病变组分别为(0.70±0.11)%、(3.80±0.55)%,乳腺癌组分别为(0.44±0.09)%、(6.51±1.10)%,乳腺癌组织中miR218-5p的阳性细胞表达率明显低于良性病变组和癌旁组,IGF-1的阳性细胞表达率明显高于良性病变组和癌旁组,良性病变组组织中miR218-5p阳性细胞表达率明显低于癌旁组,IGF-1的阳性细胞表达率明显高于癌旁组,差异均有统计学意义(P<0.05);Ⅰ级和Ⅱ级乳腺癌组织中的miR218-5p、IGF-1的阳性细胞表达率分别为(0.35±0.03)%、(6.90±1.34)%,Ⅲ级和Ⅳ级分别为(0.23±0.01)%、(11.85±2.12)%,Ⅲ级和Ⅳ级乳腺癌组织中的的miR218-5p阳性细胞率明显低于Ⅰ级和Ⅱ级乳腺癌,IGF-1阳性细胞率明显高于Ⅰ级和Ⅱ级乳腺癌,差异均有统计学意义(P<0.05);经Spearman相关性分析结果显示,miR218-5p与TNM分期呈负相关(r=-0.385,P<0.05),IGF-1与TNM分期呈正相关(r=0.693,P<0.05),乳腺癌组织中miR218-5p与 IGF-1的表达呈负相关(r=-0.326,P<0.05)。结论 乳腺癌组织中,miR218-5p表达较低,IGF-1有明显高表达,miR218-5p和IGF-1表达水平与临床病理特征有明显相关性。【关键词】 乳腺癌;良性病变组织;癌旁组织;微小RNA-218-5p;胰岛素样生长因子-1;病理特征【中图分类号】 R737.9 【文献标识码】 A 【文章编号】 1003—6350(2021)11—1415—03Expression levels of miR218-5p and IGF-1 in breast cancer tissues and their clinical significance. HUANGFang-jie, HUANG Bo, ZHANG Yi-chao, XU Hai-min. Department of Thyroid and Breast Surgery, Shenzhen Hospital(Guangming), University of Chinese Academy of Sciences, Shenzhen 518106, Guangdong, CHINA【Abstract】 Objective To study the expression levels of microRNA-218-5p (miR218-5p) and insulin-likegrowth factor-1 (IGF-1) in breast cancer tissues and their clinical significance. Methods A retrospective analysis wasperformed on 120 cases of breast cancer specimens, 60 cases of paracancerous tissue specimens (with distance from theparacancerous tissue of more than 5 cm), and 90 cases of benign breast fibroma in Department of Thyroid and BreastSurgery, Shenzhen Hospital (Guangming), University of Chinese Academy of Sciences from June 2016 to August 2019.The expression of miR218-5p and IGF-1 were compared among the three groups, and also among different TNM stagesof breast cancer. The correlation between miR218-5p, IGF-1 and TNM stages was analyzed by Spearman correlationanalysis. Results The positive expression rates of miR218-5p and IGF-1 were (1.21±0.28)% and (2.63±0.20)% in theparacancerous tissue group, (0.70±0.11)% and (3.80±0.55)% in the benign lesion group, (0.44±0.09)% and (6.51±1.10)%in the breast cancer group; the positive expression rate of miR218-5p in breast cancer group were significantly lowerthan that in benign lesion group and paracancerous tissue group; the expression rate of IGF-1 were significantly higherthan that in benign lesion group and paracancerous tissue group; the positive expression rate of miR218-5p in benign le-sion group was significantly lower than that in paracancerous tissue group, and the positive expression rate of IGF-1 inbenign lesion group was significantly higher than that in paracancerous tissue group; the differences were all statisticallysignificant (P<0.05). The positive expression rates of miR218-5p and IGF-1 were (0.35±0.03)% and (6.90±1.34)% ingrade Ⅰ and grade Ⅱ breast cancers, (0.23±0.01)% and (11.85±2.12)% in grade Ⅲ and Ⅳ breast cancers; the rate ofmiR218-5p in grade Ⅲ and grade Ⅳ breast cancer was significantly lower than that of grade Ⅰ and Ⅱ breast cancer,and the rate of IGF-1 was significantly higher than that of gradeⅠ andⅡ breast cancer; the differences were statistical-ly significant (P<0.05). Spearman correlation analysis showed that miR218-5p was negatively correlated with TNM stag-ing (r=-0.385, P<0.05), IGF-1 was positively correlated with TNM staging (r=0.693, P<0.05), and the expression of·论著·doi:10.3969/j.issn.1003-6350.2021.11.015基金项目:广东省深圳市宝安区医疗卫生基础研究项目(编号:2020JD395)
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