标题:错配修复蛋白MLH1、MSH2、PMS2、MSH6及p53在结直肠癌中的表达及意义
作者:潘思琼 1,2,陆奉科 3,李山 1 1.广西医科大学第一临床医学院,广西 南宁 530021;2.广西医科大学第四附属医院病理科,广西 柳州 545000;3.柳州市妇幼保健院输血科,广西 柳州 545000
卷次:
2021年32卷1期
【摘要】 目的 探讨结直肠癌(CRC)中错配修复蛋白(MMRP) MLH1、MSH2、PMS2、MSH6及p53的表达及其临床意义。方法 选取 2019年 1月至 2020年 6月在广西医科大学第四附属医院经手术治疗的结直肠癌患者 261例,采用免疫组化方法检测错配修复蛋白MLH1、MSH2、PMS2、MSH6、及p53在结直肠癌中的表达情况,各组中的分布差异采用χ2检验,各指标间的相关性采用Spearman等级相关。结果 261例结直肠癌患者中四种错配修复蛋白缺失者13例,微卫星不稳定性(MSI)为5.0%,其中MLH1、MSH2、PMS2、MSH6的表达缺失者分别为7例(2.7%)、3例(1.1%)、8例(3.1%)、4例(1.5%);p53的阳性表达者211例,阳性率为80.8%;MSI与性别、肿瘤细胞直径、年龄、部位、分化程度和浸润深度无关(P>0.05),与淋巴结转移相关(P<0.05);p53与性别有关(P<0.05),与年龄、部位、肿瘤细胞直径、分化程度、淋巴结是否转移和浸润深度无关(P>0.05);MSI与p53表达无相关性(r=-0.111,P>0.05);MLH1与PMS2表达呈正相关(r=0.934,P<0.05);MSH2与MSH6表达呈正相关(r=0.572,P<0.05)。结论 MSI的表达与淋巴结转移有关,与CRC的其他临床病理特征无相关性,p53与性别有关,MLH1、PMS2同时表达缺失较MSH2、MSH6多见,MSI与p53表达无相关性,两者之间可能参与CRC的不同发生、发展过程。
【关键词】 结直肠癌;p53;错配修复蛋白;免疫组化;临床病理特征;相关性
【中图分类号】 R735 【文献标识码】 A 【文章编号】 1003—6350(2021)01—0004—04
Expression and significance of mismatch repair proteins MLH1, MSH2, MSH6, PMS2, and p53 in colorectalcancer.
PAN Si-qiong 1,2, LU Feng-ke 3, LI Shan 1. 1. The First Clinical Medical College, Guangxi Medical University,Nanning 530021, Guangxi, CHINA; 2. Department of Pathology, the Fourth Affiliated Hospital of Guangxi MedicalUniversity, Liuzhou 545000, Guangxi, CHINA; 3. Department of Blood Transfusion, Liuzhou Maternity and Child HealthcareHospital, Liuzhou 545000, Guangxi, CHINA
【Abstract】 Objective To discuss the expression and significance of the mismatch repair proteins (MMRP)MLH1, MSH2, PMS2, MSH6, and p53 in colorectal cancer (CRC). Methods A total of 261 patients with colorectalcancer who were operated in the Fourth Affiliated Hospital of Guangxi Medical University from January 2019 to June2020 were selected. The expression levels of MLH1, MSH2, PMS2, MSH6, and p53 in colorectal cancer were detectedby immunohistochemical method. The distribution difference among the groups was tested by chi-square test, and Spear-man rank correlation was used for correlation analysis. Results Among 261 colorectal cancer patients, there were 13
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