标题:miR-144-3p及MAPK1 mRNA在慢性粒细胞白血病急变期单个核细胞内的表达
作者:康娅 1,杨晓雨 2,王梦昌 1 西安交通大学第一附属医院血液内科1、医疗信息管理办公室2,陕西 西安 710061
卷次:
2020年31卷21期
【摘要】 目的 观察miR-144-3p及丝裂原活化蛋白激酶1 (MAPK1) mRNA在慢性粒细胞白血病急变期单个核细胞内的表达水平。方法 收集西安交通大学第一附属医院2018年11月至2019年3月收治的慢性粒细胞白血病急变期患者10例(病例组)和正常对照组11例的外周血,每例2 mL,分离单个核细胞,利用(RT-qPCR)方法检测单个核细胞中miR-144-3p及MAPK1 mRNA的表达水平。结果 在慢性粒细胞白血病急变期单个核细胞内,病例组患者的miR-144-3p mRNA和MAPK1 mRNA表达水平分别为 0.51 [0.12~1.16]、0.91 [0.65~1.17],明显低于正常对照组的 2.45 [1.50~7.78]、2.03 [1.39~2.82],差异均有统计学意义(P<0.05)。结论 在慢性粒细胞白血病急变期单个核细胞中,miR-144-3p、MAPK1存在低表达,可为慢性粒细胞白血病干细胞研究提供新的思路。
【关键词】 慢性粒细胞白血病;急变期;miR-144-3p;丝裂原活化蛋白激酶1;干细胞
【中图分类号】 R733.72 【文献标识码】 A 【文章编号】 1003—6350(2020)21—2724—04
Expressions of miR-144-3p and MAPK1 mRNA in blastic phase chronic myeloid leukemia mononuclear cells.KANG Ya 1,YANG Xiao-yu 2, WANG Meng-chang 1.
Department of Hematology 1, Department of Health Information Services 2,the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, CHINA
【Abstract】 Objective To survey the expressions of miR-144-3p and MAPK1 mRNA in blastic phase chronicmyeloid leukemia (CML-BP) mononuclear cells. Methods The peripheral blood samples were collected from tenCML-BP patients (patient group) and eleven normal controls in the First Affiliated Hospital of Xi'an Jiaotong Universi-ty from November 2018 to March 2019, with 2 mL for each one. Mononuclear cells were acquired, and the expressionlevel of miR-144-3p and MAPK1 mRNA in CML-BP mononuclear cells was detected by real-time quantitative PCR.Results The expression of miR-144-3p and MAPK1 in blastic phase chronic myeloid leukemia mononuclear cellswere 0.51 [0.12-1.16] and 0.91 [0.65-1.17] versus corresponding 2.45 [1.50-7.78] and 2.03 [1.39-2.82] in normal con-trol cells (P<0.05). Conclusion There is the low expression of miR -144-3p and MAPK1 in CML-BP mononuclearcells. This may provide a new idea for the study of CML stem cells.
【Key words】 Chronic myeloid leukemia (CML); Blastic phase; miR-144-3p; Mitogen-activated protein kinase1(MAPK1); Stem cells
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