标题：高通量测序技术在肺部感染性疾病中的应用

      作者：肖宇 综述 肖卫 审校    长江大学附属第一医院呼吸内科，湖北 荆州 434000

      卷次： 2021年32卷5期

      【摘要】 肺部感染性疾病中，病原体的诊断对治疗有极为重要的意义。传统检测手段虽是可靠的方法，但其敏感性不足，需通过新技术，新手段来进行补充。高通量测序技术一次可以对数十万到数百万的DNA或RNA分子进行测序，具有检测范围广、检测种类多，无须培养、不受抗生素影响，指导精确治疗的优点，是避免抗生素滥用，实现呼吸系统感染性疾病精准诊疗的关键技术之一。同时，通过高通量测序技术检测痰液、肺泡灌洗液，对肺部微环境及菌群对疾病的发生、发展有了更进一步的了解。
      【关键词】 肺部感染；病原体；高通量测序；肺部微环境；菌群
      【中图分类号】 R563 【文献标识码】 A 【文章编号】 1003—6350（2021）05—0654—04

Application of high-throughput sequencing technology in pulmonary infectious diseases.
XIAO Yu, XIAO Wei.Department of Respiratory, the First Affiliated Hospital of Yangtze University, Jingzhou 434000, Hubei, CHINA【Abstract】 In pulmonary infectious diseases, the diagnosis of pathogens is extremely important for treatment. Al-though the traditional detection method is a reliable method, its sensitivity is insufficient and needs to be supplementedby new technologies and new means. High-throughput sequencing technology can sequence hundreds of thousands tomillions of DNA or RNA molecules at a time, with the advantages of wide detection range, many detection types, noneed to be cultured, not affected by antibiotics, and guiding accurate treatment. It is one of the key technologies to avoidthe abuse of antibiotics and realize the precise diagnosis and treatment of respiratory infectious diseases. At the sametime, the detection of sputum and bronchoalveolar lavage fluid by high-throughput sequencing technology has a furtherunderstanding of the pulmonary microenvironment and flora on the occurrence and development of the disease.
      【Key words】 Pulmonary infection; Pathogen; High-throughput sequencing technology; Pulmonary microenviron-ment; Bacteria·综述·doi:10.3969/j.issn.1003-6350.2021.05.032