标题：缺血再灌注心肌中补体C3的表达与补体C1抑制剂的心肌保护作用

      作者：付金容，林国生，武智晓
    （武汉大学人民医院心内三科，武汉 湖北 430060）

      卷次： 2013年24卷1期

      【摘要】 目的 研究缺血再灌注心肌中补体C3的表达以及补体1抑制剂（C1INH，C1 inhibitor）对缺血心肌
的保护作用。方法 结扎大鼠的冠状动脉左前降支 30 min，再灌注 3 h、72 h造成缺血再灌损伤模型。实验分
为假手术组、NaCl组和C1INH组（每组 5只大鼠，n=5），观察 C1INH对大鼠心功能、心肌梗死面积的影响，同
时采用免疫组化和Western blot法检测缺血心肌补体C3的表达。结果 与假手术组比较，再灌注 3 h及 72 h
后，NaCl组和C1INH组鼠心功能下降，心肌的梗死面积增加，缺血心肌补体C3的表达增加。与NaCl组比较，
再灌注 72 h后，C1INH组心功能改善，心肌的梗死面积减少[（36.28±0.99）% vs（50.58±0.24）%]，差异具有统计
学意义（P < 0.05）。同时，C1INH组缺血心肌补体C3的表达减少。结论 C1INH通过抑制缺血心肌补体C3的
表达而减少缺血再灌所致的心肌损伤，改善心功能。

      【关键词】 补体1抑制剂；缺血再灌注；补体C3

      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350(2013)01—0006—03


Expression of C3 in ischemic reperfused myocardium and the role of C1 inhibitor.
FU Jin-rong, LIN Guo-sheng,
WU Zhi-xiao. The Third Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, CHINA

【Abstract】 Objective To explore the expression of C3 in ischemic reperfused myocardium and the protec-
tive effect of C1 inhibitor (C1INH) on ischemic myocardium. Methods Rat heart ischemia/reperfusion injury was in-
duced by occluding the left anterior descending coronary artery for 30 min and reperfusion for 3 h or 72 h. C1 inhibi-
tor or NaCl was administrated intravenously 5 min before surgery. The rats were divided into three groups, the false
surgery group, the NaCl group and the C1INH group, each with 5 cases. The effect of C1INH on cardiac function and
infarct size was measured, and the expression of C3 in ischemic myocardium was detected by immunohistochemistry
and western blot. Results 3 h and 72 h after reperfusion, the NaCl group and the C1INH group had worsened cardiac
function and increased infarct size, as well as increased expression of C3, compared with the false surgery group. 72 h
after reperfusion, the C1INH group had significantly improved cardiac function and significantly reduced infarct size
[(36.28±0.99)% vs (50.58±0.24)%], compared with the NaCl group (P<0.05), as well as suppressed expression of C3.
Conclusion C1INH protects against I/R-induced myocardial injury via inhibition of C3 protein expression in isch-
emic myocardium.

      【Key words】 C1 inhibitor, Ischemia and Reperfusion, Complement C3