标题：儿茶酚抑素抑制血管钙化的作用及机制

      作者：樊小容 1，谭小青 1，张旭升 1，黄战军 1，蔡博治 2    1.深圳市龙岗区人民医院心血管内科，广东 深圳 518172；2.汕头大学医学院第一附属医院分子心脏病学实验室，广东 汕头 515041

      卷次： 2020年31卷5期

      【摘要】 目的 观察儿茶酚抑素(CST)对大鼠血管钙化的影响。方法 24只SD大鼠按随机数表法分为正常组、钙化组和钙化+CST组，每组8只。正常组大鼠在造模当天予生理盐水肌注和花生油灌胃处理，钙化组采用维生素D3和尼古丁诱导大鼠血管钙化模型，钙化+CST组给予CST 2 nmol/(kg·d)腹腔内注射 4周，检测血管钙盐沉积(Von Kossa染色)；测定大鼠主动脉钙含量(钙离子测试盒)、碱性磷酸酶(ALP)活性(碱性磷酸酶试剂盒)、主动脉组织单核细胞趋化蛋白-1 (MCP-1)和肿瘤坏死因子-α (TNF-α)含量(均为放射免疫法)。结果 维生素D3和尼古丁能够建立典型大鼠血管钙化模型；与正常组大鼠比较，Von Kossa染色示钙化组主动脉有大量黑色颗粒沉淀，经CST干预后，主动脉组织钙盐沉积明显减少；与此同时，钙化组大鼠主动脉组织的钙离子含量及ALP活性明显升高，与正常组大鼠比较，差异均有统计学意义(P<0.05)；经CST干预后，钙化+CST组大鼠的钙离子含量及ALP活性明显减轻，与钙化组大鼠比较，差异均有统计学意义(P<0.05)；钙化组大鼠血清MCP-1和TNF-α浓度明显高于正常组大鼠，差异均有统计学意义(P<0.05)；经CST干预后，钙化+CST组大鼠的血清MCP-1和TNF-α浓度较钙化组大鼠明显下降，差异均有统计学意义(P<0.05)。结论 儿茶酚抑素可抑制大鼠血管钙化，可能与下调MCP-1和TNF-α表达有关。
      【关键词】 儿茶酚抑素；血管钙化；炎症因子；单核细胞趋化蛋白-1；肿瘤坏死因子-α
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2020）05—0545—04

Effect and mechanism of catechin on vascular calcification in rat models.
FAN Xiao-rong 1, TAN Xiao-qing 1, ZHANGXu-sheng 1, HUANG Zhan-jun 1, CAI Bo-zhi 2. 1.Department of Cardiology, Longgang District People's Hospital of Shenzhen,Shenzhen 518172, Guangdong, CHINA; 2. Molecular Cardiology Laboratory, the First Affiliated Hospital of ShantouUniversity Medical College, Shantou 515041, Guangdong, CHINA
【Abstract】 Objective To observe the effect of catechin on vascular calcification in rat models. Methods Twen-ty-four rats were randomly divided into normal, calcification, and calcification plus catechin groups (8 in each group). Thenormal group was treated with normal saline and peanut oil on the day of modeling, the calcification group was induced by vi-tamin D3 plus nicotine, and the calcification plus catechin group was injected intraperitoneally with 2 nmol/(kg·d) cate-chin for 4 weeks. Vascular calcification was detected by Von Kossa staining. Calcium content and alkaline phosphatase(ALP) activity were determined by using calcium assay kit and alkaline phosphatase detection kit, respectively. The con-tents of monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) in serum were determinedby radioimmunoassay. Results Rat models of vascular calcification were established by vitamin D3 and nicotine. Com-pared with the normal group, Von Kossa staining in the calcification group showed mass black granule deposited in aor-ta, and the calcium content and ALP activity in calcified group were increased significantly (P<0.05). Meanwhile, in cal-cification group, the expression of MCP-1 and TNF-α in serum was up-regulated significantly than that in the normalgroup (P<0.05). The degree of calcification in calcification plus catechin group was improved significantly. In addi-tion, the levels of MCP-1 and TNF-α in serum decreased significantly compared with the calcification group (P<0.05). Conclusion Catechin can improve vascular calcification in rats, which may be related to the down-regulation ofMCP-1 and TNF-α expression.
      【Key words】 Catechin; Vascular calcification; Inflammatory factors; Monocyte chemoattractant protein-1(MCP-1); Tumor necrosis factor-α (TNF-α)