标题：血浆长链非编码RNA HOTAIR对预测食管癌放疗敏感性的意义

      作者：叶婷 1，李皓 1，李伟珍 1，王祝青 1，高飞 2，刘阳晨 2    扬州大学附属泰兴市人民医院检验科 1、肿瘤放疗科 2，江苏 泰兴 225400

      卷次： 2019年30卷10期

      【摘要】 目的 观察食管癌患者放疗前、后血浆长链非编码RNA HOX转录反义RNA(HOTAIR)的表达变化，并探讨其临床意义。方法 选择2017年5月至2018年12月扬州大学附属泰兴市人民医院肿瘤放疗科收治的50例食管癌患者作为研究对象(食管癌组)，另选取同期健康体检者25例作为对照组，患者采用实时定量聚合酶链反应(PCR)检测其血浆HOTAIR的水平，分析HOTAIR表达水平与患者临床病理参数及放疗疗效的关联性。结果 食管癌组患者血浆中HOTAIR水平为 0.14±0.10，明显高于健康对照组的 0.03±0.02，差异有统计学意义(P<0.05)；Ⅲ期+Ⅳ期食管癌患者放疗前血浆中HOTAIR水平为0.21±0.10，明显高于Ⅰ期+Ⅱ期患者的0.07±0.02，差异有统计学意义(P<0.05)；有淋巴转移的食管癌患者放疗后血浆中HOTAIR水平为 0.16±0.02，明显高于无淋巴转移患者的0.07±0.04，差异有统计学意义(P<0.05)；可评价疗效的 50例患者根治性放疗后血浆HOTAIR水平较放疗前升高者24例，下降者26例，升高者与下降者的放疗有效率分别为41.67%和84.62%，差异有统计学意义(P<0.05)；有效者放疗后血浆中的HOTAIR水平为0.07±0.01，明显低于其放疗前的0.25±0.06，无效者放疗后血浆中的HOTAIR水平为0.13±0.04，明显高于其放疗前的0.07±0.03)，差异均有统计学意义(P<0.05)。结论 检测食管癌患者血浆HOTAIR水平有助于放疗敏感性的早期判断，对临床上预测食管癌放疗效果有着举足轻重的作用。
      【关键词】 食管癌；放疗敏感性；长链非编码RNA；HOX转录反义RNA；血浆
      【中图分类号】 R735.1 【文献标识码】 A 【文章编号】 1003—6350（2019）10—1273—04

Predictive value of plasma lncRNA HOTAIR in radiosensitivity of esophageal cancer.
YE Ting 1, LI Hao 1, LIWei-zhen 1, WANG Zhu-qing 1, GAO Fei 2, LIU Yang-chen 2. Department of Clinical Laboratory 1, Department ofRadiotherapy 2, Taixing People's Hospital Affiliated to Yangzhou University, Taixing 225400, Jiangsu, CHINA
【Abstract】 Objective To investigate the expression and clinical value of plasma long non-coding RNA (ln-cRNA) HOTAIR level before and after radiotherapy in patients with esophageal carcinoma. Methods From May 2017to December 2018, 50 patients with esophageal carcinoma were selected as research objects from Department of Radio-therapy, Taixing People's Hospital Affiliated to Yangzhou University (esophagus cancer group), and 25 healthy individu-als were enrolled as the control group. The HOTAIR level in plasma samples were measured with quantitative real-timePCR method. The relationships among HOTAIR level expression changes, clinical stages and radiotherapy effect wereanalyzed. Results The plasma HOTAIR levels of patients with esophagus cancer was 0.14±0.10, significantly higherthan 0.03±0.02 in control group (P<0.05). The plasma HOTAIR level before radiotherapy in patients with stage Ⅲ andstageⅣ was 0.21±0.10, significantly higher than 0.07±0.02 of patients with stageⅠ and stageⅡ (P<0.05). The plasmaHOTAIR level after radiotherapy in patients with lymph node metastasis was 0.16±0.02, significantly higher than (0.07±0.04) of patients without lymph node metastasis (P<0.05). In 50 evaluated patients, the plasma HOTAIR level increasedin 24 cases but decreased in 26 cases after radical radiotherapy, and the radiotherapy efficiency was 41.67% and 84.62%,respectively (P<0.05). In effective group, the plasma HOTAIR level after radiotherapy was 0.07±0.01, significantly low-er than 0.25±0.06 before radiotherapy, while in ineffective group, the level after radiotherapy was 0.13±0.04, significant-ly higher than 0.07±0.03 before radiotherapy (P<0.05). Conclusion The plasma HOTAIR level of patients with esopha-gus cancer can help evaluate the radiosensitivity, which has great significance in predicting the efficacy of radiotherapy.
      【Key words】 Esophageal cancer; Radiosensitivity; Long non-coding RNA; HOX transcript antisense RNA; Plasma