标题：类风湿性关节炎患者外周血IL-31水平及其与抗环瓜氨酸肽抗体的相关性

      作者：钱媛 1，何应中 1，仇艳 2，石渝 3，黄俊琼 1    1.遵义医科大学附属医院检验科，贵州 遵义563000；2.云南省中医院，云南 昆明 650021；3.重庆市垫江县人民医院检验科，重庆 408300

      卷次： 2019年30卷9期

      【摘要】 目的 检测类风湿性关节炎(RA)患者外周血中白细胞介素31 (IL-31)、抗环瓜氨酸肽抗体(ACPA)水平，探讨 IL-31与ACPA的相关性。方法 选取2017年1月至2018年1月在遵义医科大学附属医院确诊的57例RA患者作为RA组，同时将研究期间于我院体检的健康体检者28例作为对照组，采集两组受检者的外周静脉血，分离血清，采用酶联免疫吸附法(ELISA)检测外周血 IL-31水平，电化学发光法检测ACPA水平，并应用采用Pearson相关分析法分析IL-31与ACPA的相关性。结果 RA组患者外周血IL-31和ACPA水平分别为(1 367.28±2 129.15) pg/mL和(323.00±379.21) U/mL，均明显高于对照组的(226.66±432.95) pg/mL和(11.28±5.16) U/mL，差异均有统计学意义(P<0.05)；IL-31与ACPA呈显著正相关(r=0.641，P<0.05)。结论 RA患者外周血 IL-31水平增高，且与ACPA呈正相关，表明 IL-31可能参与RA的发生发展，并起着重要作用。
      【关键词】 类风湿性关节炎；白介素31；抗环瓜氨酸肽抗体；炎症因子；相关性
      【中图分类号】 R593.22 【文献标识码】 A 【文章编号】 1003—6350（2019）09—1093—03

IL-31 levels in peripheral blood of patients with rheumatoid arthritis and its correlation with anti-cycliccitrullinated peptide antibody.
QIAN Yuan 1, HE Ying-zhong 1, CHOU Yan 2, SHI Yu 3, HUANG Jun-qiong 1. 1. ClinicalLaboratory, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou, CHINA; 2. Yunnan Provincial Hospital ofTraditional Chinese Medicine, Kunming 650021, Yunnan, CHINA; 3. Clinical Laboratory, Dianjiang People's Hospital ofChongqing, Dianjiang 408300, Chongqing, CHINA
【Abstract】 Objective To detect the levels of interleukin 31 (IL-31) and anti-cyclic citrullinated peptide anti-body (ACPA) in peripheral blood of patients with rheumatoid arthritis (RA), and to explore the correlation betweenIL-31 and ACPA. Methods From January 2017 to January 2018, 57 RA patients diagnosed in the Affiliated Hospitalof Zunyi Medical University were enrolled in RA group, and 28 healthy people who underwent physical examination inour hospital during the study period were selected as a control group. Peripheral venous blood of the two groups was col-lected and serum was separated. IL-31 levels in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA), and the ACPA levels was detected by electrochemiluminescence. The correlation between IL-31 and ACPAwas analyzed by Pearson correlation analysis. Results The levels of IL-31 and ACPA in the peripheral blood of the RAgroup were (1 367.28±2 129.15) pg/mL and (323.00±379.21) U/mL, respectively, which were significantly higher thancorresponding (226.66±432.95) pg/mL and (11.28±5.16) U/mL of the control group, and the differences were statistical-ly significant (P<0.05). IL-31 was significantly positively correlated with ACPA (r=0.641, P<0.05). Conclusion Thelevel of IL-31 in peripheral blood of patients with RA is increased and positively correlated with ACPA, indicating thatIL-31 may be involved in the development of RA and plays an important role.
      【Key words】 Rheumatoid arthritis; Interleukin 31; Anti-cyclic citrullinated peptide antibody (ACPA); Inflamma-tory factor; Correlation