标题：结直肠癌微血管、淋巴管以及血管生成拟态与其临床病理特征的关系

      作者：吴共发 1，邱丽浈 2，黄绮亭 1，曾宇婷 1，刘钰君 1，李海刚 1，3    1.广州市增城区人民医院病理科，广东 广州 511300；2.广州市增城区人民医院体检中心，广东 广州 511300；3.中山大学孙逸仙纪念医院病理科，广东 广州 510120

      卷次： 2019年30卷9期

      【摘要】 目的 研究结直肠癌中微血管密度(MVD)、淋巴管密度(LVD)和血管生成拟态(VM)与其临床病理特征的关系。方法 选取广州市增城区人民医院2015年3月至2017年12月期间手术切除的结直肠癌标本77例作为研究对象，用免疫组织化学CD34染色检测MVD，免疫组织化学D2-40染色检测LVD，利用CD34和PAS双重染色检测VM，分析MVD、LVD和VM与结直肠癌临床病理特征之间的关系。结果 结直肠癌浸润边缘的MVD为45.1±19.7，明显高于正常结直肠组织的30.4±11.2；结直肠癌浸润边缘的LVD为42.5±15.6，明显高于正常结直肠组织的21.6±12.3，差异均有统计学意义(P<0.05)；结直肠癌浸润边缘的MVD平均值在不同肿瘤大小、肿瘤分化程度、临床分期以及有无神经侵犯间的两两比较，差异均有统计学意义(P<0.05)；有淋巴结转移者的肿瘤浸润边缘的LVD为52.5±21.8，明显高于无淋巴结转移者的30.1±16.4，差异有统计学意义(P<0.05)；LVD在不同年龄、性别、肿瘤分化程度、肿瘤大小、临床分期患者中比较，差异均无统计学意义(P>0.05)；结直肠癌组织中出现VM的例数在不同肿瘤大小、肿瘤分化程度、临床分期以及有无神经侵犯者间比较差异均有统计学意义(P<0.05)；相关性分析结果显示，MVD、LVD与VM呈显著密切正相关(r均>0.5，P<0.01)。结论 结直肠癌中MVD、LVD和VM与其不良临床病理特征有密切关系，对判断结直肠癌侵袭、转移乃至预后可能有重要意义。
      【关键词】 结直肠癌；微血管密度；淋巴管密度；血管生成拟态；病理学
      【中图分类号】 R735 【文献标识码】 A 【文章编号】 1003—6350（2019）09—1089—04

Relationship between microvessels, lymphatic vessels, vasculogenic mimicry of colorectal carcinoma and itsclinicopathological features.
WU Gong-fa 1, QIU Li-zhen 2, HUANG Qi-ting 1, ZENG Yu-ting 1, LIU Yu-jun 1, LIHai-gang 1, 3. 1. Department of Pathology, Zengcheng District People's Hospital of Guangzhou, Guangzhou 511300, Guangdong,CHINA; 2. Health Examination Center, Zengcheng District People's Hospital of Guangzhou, Guangzhou 511300,Guangdong, CHINA; 3. Department of Pathology, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou510120, Guangzhou, CHINA
【Abstract】 Objective To study the relationship between microvessel density (MVD), lymphatic vessel density(LVD), vasculogenic mimicry (VM) and its clinicopathological features in colorectal carcinoma (CRC), and provide ref-erence for clinical diagnosis and treatment. Methods Seventy-seven cases of colorectal cancer specimens surgically re-sected from March 2015 to December 2017 in Zengcheng District People's Hospital of Guangzhou were selected as sub-jects. MVD was detected by immunohistochemical CD34 staining, LVD was detected by immunohistochemical D2-40staining, and VM was detected by VM34 and PAS double staining. The relationship between MVD, LVD and VM andclinicopathological features of colorectal cancer was analyzed. Results The MVD of the invasive edge of colorectalcancer was 45.1±19.7, which was significantly higher than 30.4±11.2 of normal colorectal tissue. The LVD of the infil-trating edge of colorectal cancer was 42.5±15.6, which was significantly higher than 21.6±12.3 of normal colorectal tis-sue (P<0.05); the mean value of MVD in the invasive edge of colorectal cancer was compared between different tumorsize, tumor differentiation degree, clinical stage and presence or absence of neurological invasion, and all the differenceswere statistically significant (P<0.05). The LVD of the tumor infiltration margin was 52.5±21.8, which was significantlyhigher than 30.1±16.4 of non-lymph node metastasis (P<0.05); LVD had no significant difference in age, gender, tumordifferentiation, tumor size, clinical stage, etc (P>0.05); the number of VMs in colorectal cancer tissues was compared indifferent tumor size, tumor differentiation degree, clinical staging and presence or absence of neurological invasion, andall the differences were statistically significant (P<0.05). Correlation analysis showed that MVD, LVD and VM were sig-