标题：一起由产A型肠道毒素金黄色葡萄球菌引起的食物中毒实验室分析

      作者：吴南卫，朱兰兰，尹江源，黄春梅，邓瑶，邓兴霞    三亚市疾病预防控制中心，海南 三亚 572000

      卷次： 2019年30卷8期

      【摘要】 目的 对三亚市某幼儿园一起细菌性食物中毒进行实验室分析，为食物中毒处置提供依据。方法 根据GB/T4789《食品微生物学检验》对所采样品进行常见致病菌培养，采取实时荧光PCR方法对检出的金黄色葡萄球菌进行A~E型肠毒素检测。结果 从8份留样食品、5份患者肛拭子检出产A型肠毒素的金黄色葡萄球菌，1份患者呕吐物检出产C型和D型肠毒素的金黄色葡萄球菌。结论 该起食物中毒事件主要由产A型肠毒素的金黄色葡萄球菌引起。建议加强学校、幼儿园餐饮食品卫生监督和采购环节的管理，提高老师卫生意识，防止食物中毒的发生。
      【关键词】 肠毒素；金黄色葡萄球菌；食物中毒；分离培养；实时荧光PCR
      【中图分类号】 R378.1+1 【文献标识码】 A 【文章编号】 1003—6350（2019）08—1047—03

Laboratory analysis of a food poisoning case caused by Staphylococcus aureus producing enterotoxin A.
WUNan-wei, ZHU Lan-lan, YIN Jiang-yuan, HUANG Chun-mei, DENG Yao, DENG Xing-xia. Sanya Center for DiseasePrevention and Control, Sanya 572000, Hainan, CHINA
【Abstract】 Objective To detect the cause of bacterial food poisoning in a kindergarten in Sanya through labo-ratory analysis, so as to provide basis for the handling of food poisoning events. Methods The samples collected fromfood poisoning were tested for pathogenic bacteria according to GB/T4789 Food Microbiology Test, and Staphylococcalenterotoxins A to E were detected by the real-time fluorescent PCR. Results Staphylococcus aureus strains were detect-ed from 8 preserved foods and 5 patients’anal swabs, and identified as Staphylococcus aureus producing enterotoxin A.Staphylococcal enterotoxin C and D was found in one patient's vomit. Conclusion The food poisoning incident wasmainly caused by Staphylococcus aureus producing enterotoxin A. It is recommended to strengthen the supervision offood hygiene and procurement links in schools and kindergartens, and to improve teachers' awareness of hygiene, so asto prevent food poisoning.
      【Key words】 Enterotoxin; Staphylococcus aureus; Food poisoning; Isolation and identification; Real-time PCR·短篇论著·doi:10.3969/j.issn.1003-6350.2019.08.028