标题：合生元在1,2-二甲基肼诱导大鼠结肠损伤过程中的抗氧化作用

      作者：杜艳平，区俊文，朱薪宇，张剑锋，黄吾瑞    广州中医药大学祈福医院营养科，广东 广州 510000

      卷次： 2019年30卷2期

      【摘要】 目的 探讨合生元在 1,2-二甲基肼(DMH)致大鼠结肠损伤过程中对结肠病理的影响，并从抗氧化作用探讨其机制。方法 选取 30只 SD大鼠，按照随机区组的方法分为对照组、DMH组和合生元干预组，每组10只。除对照组外，另外两组以 0.4%的DMH溶液进行颈部皮下注射，同时合生元干预组给予 5×108 CFU/kg的合生元灌胃；实验第 14天，处死所有大鼠，结肠切片用HE染色观察病理改变；按照试剂盒的方法测定血浆内毒素含量、结肠匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)活性、总抗氧化能力(T-AOC)等指标。结果 DMH组和合生元干预组大鼠体质量增长缓慢，在实验第 7天和第 14天均低于对照组，差异均有统计学意义(P<0.05)；实验第 14天，DMH组和合生元干预组大鼠结肠见慢性炎症细胞浸润，结肠湿重分别为(63.21±4.392) g和(64.75±2.83) g，均低于对照组的(70.16±3.51) g，差异均具有统计学意义(P<0.05)；合生元干预组大鼠血浆内毒素含量为(40.32±6.33) EU/L，低于DMH组的(52.55±4.62) EU/L，但两组均高于对照组的(30.02±3.08) EU/L，差异均具有统计学意义(P<0.05)；检测结肠匀浆MDA、SOD、T-AOC、MPO，结果显示，对照组分别为(1.595±0.13) nmol/mg prot、(31.41±3.86) U/mg prot、(0.545±0.13) U/mg prot、(0.629±0.11) U/mg prot，DMH组分别为(2.024±0.21) nmol/mg prot、(31.51±4.89) U/mg prot、(0.785±0.09) U/mg prot、(0.806±0.12) U/mg prot，合生元干预组分别为(1.987±0.17) nmol/mg prot、(33.28±3.73) U/mg prot、(0.713±0.11) U/mg prot、(0.733±0.10) U/mg prot，其中给予DMH的两组大鼠的MDA、T-AOC均高于对照组，DMH组大鼠的MPO高于对照组和合生元干预组，差异均有统计学意义(P<0.05)；但三组大鼠的结肠匀浆 SOD含量比较差异均无统计学意义(P>0.05)。结论 合生元能够减弱DMH对大鼠结肠的损伤，具有抗脂质过氧化的作用，在DMH诱导的结肠损伤过程中起到一定的保护作用。
      【关键词】 合生元；结肠损伤；氧化应激；抗氧化作用；肠道微生态
      【中图分类号】 R-332 【文献标识码】 A 【文章编号】 1003—6350（2019）02—146—04A

ntioxidant effect of synbiotics on colonic injury induced by 1,2-dimethylhydrazine in rats.
DU Yan-ping, OUJun-wen, ZHU Xin-Yu, ZHANG Jian-feng, HUANG Wu-rui. Department of Nutrition, Clifford Hospital, GuangzhouUniversity of Chinese Medicine, Guangzhou 510000, Guangdong, CHINA
【Abstract】 Objective To discuss the effects of synbiotics on pathology damage of colon in rats with colonic in-jury induced by 1,2-dimethylhydrazine (DMH), and to investigate the mechanism from antioxidation. Methods ThirtySD rats were selected and randomly divided into control group, DMH group and syngenetic intervention group accord-ing to the random group method, with 10 rats in each group. Except the control group, subcutaneous injection into theneck was performed in the other two groups with a solution of 0.4% DMH, and in the syngenetic intervention group withan oral gavage of 5×108 CFU/kg. On the 14th day of the experiment, all rats were killed and the pathology change of co-lon slices was observed by HE staining. Endotoxin content in plasma, and colon homogenate indicators such as contentof malonaldehyde (MDA), enzymatic activity of superoxide dismutase (SOD) and myeloperoxidase (MPO), and totalantioxidant capacity (T-AOC) were measured according to the kit methods. Results The body weights of rats in theDMH group and the syngenetic intervention group increased slowly, which was lower than that of the control group onthe 7th and 14th day of the experiment, and the differences were statistically significant (P<0.05). On the 14th day of theexperiment, chronic inflammatory cell infiltration was observed in the colon of rats in the DMH group and the syngenet-ic intervention group. The wet colon weight of colon in the two groups was respectively (63.21±4.392) g and (64.75±2.83) g, which was lower than (70.16±3.51) g in the control group, and both differences were statistically significant(P<0.05). The plasma endotoxin content of the rats in the syngenetic intervention group was lower than that in theDMH group: (40.32±6.33) EU/L vs (52.55±4.62) EU/L, but the content of both groups was respectively higher than(30.02±3.08) EU/L in the control group with statistically significant differences (P<0.05). The contents of MDA, SOD,T-AOC and MPO in the colonic homogenate in the control group were respectively (1.595 ± 0.13) nmol/mg prot,(31.41±3.86) U/mg prot, (0.545±0.13) U/mg prot, and (0.629±0.11) U/mg prot; (2.024±0.21) nmol/mg prot, (31.51±4.89) U/mg prot), (0.785±0.09) U/mg prot, and (0.806±0.12) U/mg prot in the DMH group; and (1.987±0.17) nmol/mg prot,doi:10.3969/j.issn.1003-6350.2019.02.003基金项目：广东省广州市番禺区科技计划项目(编号：2014-Z03-49)