标题：LncRNA AC008871.1的鉴定及在肝癌中的表达

      作者：陈安宁，陈思宇，宋伟，王鹏飞，刘玲珑，姚清媚，周素芳    广西医科大学基础医学院，广西 南宁 530021

      卷次： 2019年30卷2期

      【摘要】 目的 探讨长链非编码RNA AC008871.1的鉴定及在肝癌中的表达，并预测其靶基因。方法 应用USCS数据库检索其位置、CPC数据库分析其编码能力、Ensembl数据库预测其靶基因；再通过实时荧光定量PCR技术分析AC008871.1在4种肝癌细胞系(SK-Hep1、HepG2、Huh7、97L)与正常细胞系(L-02)中的差异表达，以及在肝癌组织与癌旁组织中的差异表达，并分析其与临床数据的关系。结果 经数据库检索，AC008871.1位于5号染色体长臂，酪氨酸激酶FER基因3号至4号外显子之间的3号内含子的反义链上，其编码能力很弱，为非编码RNA，预测其靶基因可能为FER；经荧光定量分析，AC008871.1在不同肝癌细胞系中相对表达量均低于正常细胞，与L-02的(1.00±0.00)相比，在 SK-Hep1、HepG2、Huh7、97L中的相对表达量依次是(0.32±0.22)、(0.49±0.08)、(0.83±0.03)、(0.42±0.03)，差异均有统计学意义(P<0.05)；肝癌组织中AC008871.1的相对表达量均低于癌旁组织[(0.29±0.21) vs1.00]，差异有统计学意义(P<0.05)；有吸烟史的肝癌组织中，AC008871.1的基因表达水平为(0.40±0.22)，明显高于无吸烟的肝癌组织的(0.17±0.10)，差异有统计学意义(P<0.05)；HBEAb正常的肝癌组织中，AC008871.1的基因表达水平为(0.41±0.20)，明显高于不正常的肝癌组织的(0.16±0.12)，差异有统计学意义(P<0.05)。结论 AC008871.1差异表达可能与肝癌的发生、发展有密切联系，有望作为新的肝癌预后标志物和治疗靶点。
      【关键词】 长链非编码RNA；实时荧光定量PCR；AC008871.1；肝癌；酪氨酸激酶
      【中图分类号】 R735.7 【文献标识码】 A 【文章编号】 1003—6350（2019）02—0135—05

Identification of LncRNA AC008871.1 and its expression in liver cancer.
CHEN An-ning, CHEN Si-yu, SONG Wei,WANG Peng-fei, LIU Ling-long, YAO Qing-mei, ZHOU Su-fang. Department of Biochemistry, School of Basic MedicalSciences, Guangxi Medical University, Nanning 530021, Guangxi, CHINA
【Abstract】 Objective To identify the long-chain non-coding RNA AC008871.1 and its expression in liver can-cer, and to predict its target gene. Methods The USCS database was used to search its location, the CPC database wasused to analyze its coding ability, and the Ensembl database was used to predict its target gene. Then, real-time quantita-tive PCR was used to evaluate the differential expression of AC008871.1 in four hepatocellular carcinoma cell lines(SK-Hep1, HepG2, Huh7, 97L) and in normal cell lines (L-02), as well as the differential expression in liver cancer tis-sues and adjacent tissues, and to analyze their relationship with clinical data. Results According to the database search,AC008871.1 is located on the antisense strand of the 3rd intron between the long arm of chromosome 5 and the exon 3to 4 of the tyrosine kinase FER gene, which is non-coding RNA and has weak coding ability, and the target gene may bepredicted to be FER. By fluorescence quantitative analysis, the relative expression of AC008871.1 in different liver can-cer cell lines was lower than that of normal cells: compared with L-02 (1.00 ± 0.00), the relative expression levels inSK-Hep1, HepG2, Huh7, and 97L were 0.32±0.22, 0.49±0.08, 0.83±0.03, and 0.42±0.03, respectively; the differencewas statistically significant (P<0.05). The relative expression of AC008871.1 in liver cancer tissues was lower than thatin adjacent tissues (0.29±0.21 vs 1.00), and the difference was statistically significant (P<0.05). In the liver cancer tissueswith smoking history, the gene expression level of AC008871.1 (0.40 ± 0.22) was significantly higher than that ofnon-smoking liver cancer tissues (0.17±0.10), and the difference was statistically significant (P<0.05). In HBEAb nor-mal liver cancer tissues, the gene expression level of AC008871.1 (0.41±0.20) was significantly higher than that of ab-normal liver cancer tissues (0.16±0.12), and the difference was statistically significant (P<0.05). Conclusion The dif-ferential expression of AC008871.1 may be closely related to the occurrence and development of liver cancer, and it isexpected to be a new prognostic marker and therapeutic target for liver cancer.
      【Key words】 Long-chain non-coding RNA (LncRNA); Real-time quantitative PCR (qRT-PCR); AC008871.1;Liver cancer; Tyrosine kinase