标题：乌司他丁对人心脏微循环内皮细胞释放炎性因子的作用

      作者：闫庆峰，赵映，刘苏，刘运仲    (海南医学院第一附属医院心胸外科，海南 海口 570102)

      卷次： 2018年29卷22期

      【摘要】 目的 探讨乌司他丁(UTI)对人心脏微循环内皮细胞(HCMEC)释放炎性因子的影响。方法 将体外培养的HCMEC细胞按以下分组方法进行实验：①对照组，在HCMEC细胞培养体系中加入2 mL的体外循环血清；② UTI-H组，HCMEC细胞培养体系+2 mL体外循环血清+1 000单位/mL的UTI；③ UTI-M组，HCMEC细胞培养体系+2 mL体外循环血清+500单位/mL的UTI；④ UTI-L组，HCMEC细胞培养体系+2 mL体外循环血清+100单位/mL的UTI。每组各9个培养皿。检测各组细胞培养液上清液中肿瘤坏死因子α (TNF-α)、白细胞介素10 (IL-10)、核转录因子κB (NF-κB)的含量。结果 (1) UTI-H组、UTI-M组和UTI-L组细胞培养液上清液中的NF-κB水平分别为(0.04±0.005) ng/mL、(0.06±0.002) ng/mL和(0.07±0.003) ng/mL，明显低于对照组的(0.10±0.006) ng/mL，且UTI-H组NF-κB水平明显低于UTI-M组和UTI-L组，差异均有统计学意义(P<0.05)；(2) UTI-L组、UTI-M组和UTI-H组细胞培养液上清液中的TNF-α水平分别为(0.71±0.09) ng/L、(0.66±0.05) ng/L、(0.32±0.04) ng/L，明显低于对照组的(1.45±0.06) ng/L，且UTI-H组的TNF-α水平明显低于UTI-M组和UTI-L组，差异均有统计学意义(P<0.05)；(3) UTI-L组、UTI-M组和UTI-H组细胞培养液上清液中的 IL-10水平分别为(1.29±0.12) ng/L、(2.33±0.15) ng/L、(4.42±0.36) ng/L，明显高于对照组的(0.76±0.09) ng/L，UTI-H组的 IL-10水平明显高于UTI-M组和UTI-L组，UTI-M组 IL-10明显高于UTI-L组，差异均有统计学意义(P<0.05)；(4) UTI-L组和UTI-M组的 NF-κB和TNF-α水平比较，差异均无统计学意义(P>0.05)。结论 乌司他丁能明显降低HCMEC细胞释放TNF-α、NF-κB水平和提升HCMEC细胞释放 IL-10因子水平。
      【关键词】 体外循环；人心脏微血管内皮细胞；乌司他丁；炎性因子
      【中图分类号】 R541 【文献标识码】 A 【文章编号】 1003—6350（2018）22—3109—03

Effects of Ustastatin on the release of inflammatory cytokines in human heart microcirculation endothelial cells.YAN Qing-feng, ZHAO Ying, LIU Su, LIU Yun-zhong.
Department of Cardiothoracic Surgery, the First Affiliated Hospital ofHainan Medical University, Haikou 570102, Hainan, CHINA
【Abstract】 Objective To investigate the effect of Ustastatin (UTI) on the inflammatory factors of human cardi-ac microcirculation endothelial cells (HCMEC). Methods HCMEC were cultured in vitro and divided into four groups:the control group (2 mL extracorporeal circulation serum was added to HCMEC cell culture system); the high-dose UTIgroup (2 mL extracorporeal circulation serum+1 000 units/mL UTI was added to HCMEC cell culture system); the medi-um-dose UTI group (2 mL extracorporeal circulation serum +500 units/mL UTI was added to HCMEC cell culture sys-tem); the low-dose UTI group (2 mL extracorporeal circulation serum+100 units/mL UTI was added to HCMEC cell cul-ture system). The levels of tumor necrosis factor (TNF-α), interleukin-10 (IL-10), nuclear factor-kappa B (NF-κB)were measured and analyzed in the each group. Results (1) The levels of NF-κB of the high-dose UTI group, the mid-dle-dose UTI group, and the low-dose UTI group were (0.04±0.005) ng/mL, (0.06±0.002) ng/mL, and (0.07±0.003) ng/mL,significantly lower than (0.10±0.006) ng/mL in the control group, and the levels of NF-κB in the high-dose UTI groupwas significantly lower than that in the middle-dose UTI group and the low-dose UTI group, all with statistically signifi-cant differences (P<0.05). (2) The levels of TNF-α of the low-dose UTI group, the middle-dose UTI group and thehigh-dose UTI group were (0.71±0.09) ng/L, (0.66±0.05) ng/L, (0.32±0.04) ng/L, significantly lower than (1.45±0.06) ng/Lin the control group, and the levels of TNF-α in the high-dose UTI group was significantly lower than those in the mid-dle-dose UTI group and the low-dose UTI group, all with statistically significant differences (P<0.05). (3) The levels ofIL-10 of the low-dose UTI group, the middle-dose UTI group and the high-dose UTI group were (1.29 ± 0.12) ng/L,(2.33±0.15) ng/L, (4.42±0.36) ng/L, significantly higher than (0.76±0.09) ng/L of the control group. The levels of IL-10in the high-dose UTI group was significantly higher than those in the middle-dose UTI group and the low-dose UTIgroup, and the levels of IL-10 in the middle-dose UTI group was higher than that in the low-dose UTI group, all with sta-tistically significant differences (P<0.05). (4) There was no significant difference between the middle-dose UTI groupand the low-dose UTI group in the levels of NF-κB and TNF-α (P>0.05). Conclusion Ulinastatin significantly re-duced TNF-α and NF-κB levels and increased IL-10 level in HCMEC cells.
      【Key words】 Extracorporeal circulation; Human microvascular endothelial cells; Ulinastatin; Inflammatory cytokines·论 著·基金项目：海南省海口市重点科技